Cryo electron microscopy of Mce1 transporter from Mycobacterium smegmatis
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
Research Square (2023) [ DOI: 10.21203/rs.3.rs-2412186/v1 ]
Image sets:
Structure of Mce1-LucB complex from Mycobacterium smegmatis (Map1)
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8fed
Q-score: 0.524
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Unknown ligand (Ligand)
- Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Transmembrane protein (20 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) bound to accessory factor lucb (Complex from Mycolicibacterium smegmatis MC2 155)
Structure of Mce1 transporter from Mycobacterium smegmatis in the absence of LucB (Map2)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8fee
Q-score: 0.502
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Unknown ligand (Ligand)
- Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8fef
Q-score: 0.564
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Unknown ligand (Ligand)
- Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Local refinement of MceG in Msmeg Mce1 transporter (Map0a)
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Virulence factor mce family protein mce1d (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1e (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1f (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Atp-binding protein mceg (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1c (Protein from Mycolicibacterium smegmatis MC2 155)
Local refinement of YrbE and MCE ring in Msmeg Mce1 transporter (Map0b)
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Virulence factor mce family protein mce1d (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1e (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1f (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Atp-binding protein mceg (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1c (Protein from Mycolicibacterium smegmatis MC2 155)
Local refinement of MCE ring and needle in Msmeg Mce1 transporter (Map0c)
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Virulence factor mce family protein mce1d (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1e (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1f (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Atp-binding protein mceg (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1c (Protein from Mycolicibacterium smegmatis MC2 155)
Local refinement of MCE needle and portal in Msmeg Mce1 transporter (Map0d)
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Virulence factor mce family protein mce1d (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1e (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1f (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Atp-binding protein mceg (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1c (Protein from Mycolicibacterium smegmatis MC2 155)
Raw, consensus refinement of Msmeg Mce1 transporter (Map0e)
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Virulence factor mce family protein mce1d (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1e (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1f (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Atp-binding protein mceg (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1c (Protein from Mycolicibacterium smegmatis MC2 155)
Local refinement of MceG in Msmeg Mce1-LucB complex (Map1a)
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]
- Virulence factor mce family protein mce1d (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce binding protein lucb/msmeg_3032 (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1e (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1f (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1b (Protein from Mycolicibacterium smegmatis MC2 155)
- Atp-binding protein mceg (Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter permease protein yrbe1a (Protein from Mycolicibacterium smegmatis MC2 155)
- Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) bound to accessory factor lucb (Complex from Mycolicibacterium smegmatis MC2 155)
- Virulence factor mce family protein mce1c (Protein from Mycolicibacterium smegmatis MC2 155)