TpeA bound closed MthK-A88F mutant in nanodisc
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8fz7
Q-score: 0.511
Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM
Nat Chem Biol (2024) 20 pp. 52-61 [ DOI: doi:10.1038/s41589-023-01406-2 Pubmed: 37653172 ]
- Potassium ion (39 Da, Ligand)
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- 1-(tripentyl-$l^{4}-azanyl)pentane (298 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Tpea bound mthk a88f mutant in 0 ca2+ (220 kDa, Complex from Methanothermobacter thermautotrophicus)
MthK-A90L mutant in closed state with 0 Ca2+
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8djb
Q-score: 0.471
Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM
Nat Chem Biol (2024) 20 pp. 52-61 [ DOI: doi:10.1038/s41589-023-01406-2 Pubmed: 37653172 ]
- Potassium ion (39 Da, Ligand)
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- Mthk a90l mutant in 0 ca2+ (220 kDa, Complex from Methanothermobacter thermautotrophicus)
Blocker-free closed MthK channel in nanodisc
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 5bki
Q-score: 0.566
Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM
Nat Chem Biol (2023) [ DOI: doi:10.1038/s41589-023-01406-2 Pubmed: 37653172 ]
- Potassium ion (39 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Cadmium ion (112 Da, Ligand)
- Mthk in nanodisc (Complex from Methanothermobacter thermautotrophicus)
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- Water (18 Da, Ligand)
TPeA-bound closed MthK channel in nanodisc
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 5bkj
Q-score: 0.495
Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM
Nat Chem Biol (2023) [ DOI: doi:10.1038/s41589-023-01406-2 Pubmed: 37653172 ]
- Mthk (Complex from Methanothermobacter thermautotrophicus)
- Potassium ion (39 Da, Ligand)
- 1-(tripentyl-$l^{4}-azanyl)pentane (298 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- Water (18 Da, Ligand)
bbTBA-bound closed MthK channel in nanodisc
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 5bkk
Q-score: 0.508
Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM
Nat Chem Biol (2023) [ DOI: doi:10.1038/s41589-023-01406-2 Pubmed: 37653172 ]
- (~{r})-phenyl-[4-[(tributyl-$l^{4}-azanyl)methyl]phenyl]methanol (382 Da, Ligand)
- Mthk (Complex from Methanothermobacter thermautotrophicus)
- Potassium ion (39 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
Calcium-bound MthK open-inactivated state 3
Resolution: 5.0 Å
EM Method: Single-particle
Fitted PDBs: 6u6h
Q-score: 0.278
Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM
Nature (2020) 580 pp. 288-293 [ Pubmed: 32269335 DOI: doi:10.1038/s41586-020-2116-0 ]
Calcium-bound MthK gating ring state 2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6u5n
Q-score: 0.558
Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM
Nature (2020) 580 pp. 288-293 [ Pubmed: 32269335 DOI: doi:10.1038/s41586-020-2116-0 ]
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Calcium-bound mthk gating ring (200 kDa, Complex from Methanothermobacter thermautotrophicus)
Calcium-bound MthK gating ring state 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6u5p
Q-score: 0.541
Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM
Nature (2020) 580 pp. 288-293 [ Pubmed: 32269335 DOI: doi:10.1038/s41586-020-2116-0 ]
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Calcium-bound mthk gating ring (Complex from Methanothermobacter thermautotrophicus)
Calcium-bound MthK closed state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6u5r
Q-score: 0.494
Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM
Nature (2020) 580 pp. 288-293 [ Pubmed: 32269335 DOI: doi:10.1038/s41586-020-2116-0 ]
- Calcium-gated potassium channel mthk (37 kDa, Protein from Methanothermobacter thermautotrophicus)
- Calcium-bound mthk (Complex from Methanothermobacter thermautotrophicus)
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
Calcium-bound MthK open-inactivated state 1
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6u68
Q-score: 0.338
Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM
Nature (2020) 580 pp. 288-293 [ Pubmed: 32269335 DOI: doi:10.1038/s41586-020-2116-0 ]