Substrate-free AAA single-chained ClpX∆N protein in a complex with ClpP peptidase
Ghanbarpour A, Cohen SE, Fei X, Sauer RT, Davis JH
Nat Commun (2023) [ DOI: 10.1101/2022.08.27.505532 ]
Image sets:
Full-length ClpX AAA protein in a complex with ClpP peptidase
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8et3
Q-score: 0.382
Ghanbarpour A, Fei X, Baker TA, Davis JH, Sauer RT
PNAS (2023) 120 pp. e2219044120-e2219044120 [ DOI: doi:10.1073/pnas.2219044120 Pubmed: 36730206 ]
- Stringent starvation protein b (18 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (46 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Green fluorescent protein (30 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Clpxp aaa protease complex bound with sspb and gfp-ssra (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class IIa
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7uiv
Q-score: 0.416
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class IIb
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7uiw
Q-score: 0.387
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class I
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7uix
Q-score: 0.449
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class IIc
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7uiz
Q-score: 0.453
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class IIIb
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 7uj0
Q-score: 0.445
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class IIIa
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7uiy
Q-score: 0.463
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)