Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8et3
Q-score: 0.382
Ghanbarpour A, Fei X, Baker TA, Davis JH, Sauer RT
PNAS (2023) 120 pp. e2219044120-e2219044120 [ Pubmed: 36730206 DOI: doi:10.1073/pnas.2219044120 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Green fluorescent protein (30 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Stringent starvation protein b (18 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease atp-binding subunit clpx (46 kDa, Protein from Escherichia coli)
- Clpxp aaa protease complex bound with sspb and gfp-ssra (Complex from Escherichia coli)
ClpP and ClpX IGF loop in ClpX-ClpP complex bound to ssrA tagged GFP
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 6wr2
Q-score: 0.518
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ Pubmed: 33089779 DOI: doi:10.7554/eLife.61496 ]
- Clpx-clpp-gfpssra-atp/atprs (Complex from Escherichia coli)
- Water (18 Da, Ligand)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli (strain K12))
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli (strain K12))
ClpX-ClpP complex bound to GFP-ssrA, recognition complex
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 6wrf
Q-score: 0.414
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ Pubmed: 33089779 DOI: doi:10.7554/eLife.61496 ]
- Clpx-clpp-gfpssra-atp/atprs complex during target recognition (Complex from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Water (18 Da, Ligand)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli (strain K12))
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Green fluorescent protein (30 kDa, Protein from Aequorea victoria)
ClpX-ClpP complex bound to ssrA-tagged GFP, intermediate complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 6wsg
Q-score: 0.406
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ Pubmed: 33089779 DOI: doi:10.7554/eLife.61496 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Clpx-clpp-gfpssra-atp/atprs (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Green fluorescent protein, + ssra tag (30 kDa, Protein from synthetic construct)
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)