afTMEM16 in C22 lipid nanodiscs with MSP2N2 scaffold protein in the presnece of Ca2+
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rwj
Q-score: 0.484
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Plasma membrane channel protein (aqy1), putative (84 kDa, Protein from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100))
- Aftmem16 scramblase c22 lipid nanodiscs with msp2n2 scaffold in the presence of ca2+ (Complex from Aspergillus fumigatus)
afTMEM16 in C22 lipid nanodiscs with MSP1E3 scaffold protein in the presnece of Ca2+
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7rx2
Q-score: 0.632
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 scramblase c22 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+ (Complex from Aspergillus fumigatus)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
afTMEM16 in C14 lipid nanodiscs with MSP1E3 scaffold protein in the absence of Ca2+
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7rx3
Q-score: 0.533
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
- Aftmem16 scramblase in c14 lipid nanodiscs with msp1e3 scaffold in the absence of ca2+ (Complex from Aspergillus fumigatus)
afTMEM16 DE/AA mutant in C14 lipid nanodiscs in the presence of Ca2+
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7rxa
Q-score: 0.558
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- Aftmem16 de/aa mutant in c14 lipid nanodiscs in the presence of ca2+ (Complex from Aspergillus fumigatus)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 7rxb
Q-score: 0.595
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 lipid scramblase in c18 lipid nanodiscs in the absence of ca2+ (Complex from Aspergillus fumigatus)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
afTMEM16 in C18 lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+, full dimer
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 7rxg
Q-score: 0.648
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Aftmem16 scramblase c18 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+, full dimer (Complex from Aspergillus fumigatus)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7rxh
Q-score: 0.556
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ Pubmed: 35562175 DOI: doi:10.1038/s41467-022-30300-z ]
- Water (18 Da, Ligand)
- Aftmem16 scramblase c18 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+, monomer with additional lipids (Complex from Aspergillus fumigatus)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
- Calcium ion (40 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)