Dephosphorylated CFTR in 1:1 complex with PKA-C (site I)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 9dw5
Q-score: 0.397
Fiedorczuk K, Iordanov I, Mihalyi C, Szollosi A, Csanady L, Chen J
PNAS (2024) 121 pp. e2409049121-e2409049121 [ DOI: doi:10.1073/pnas.2409049121 Pubmed: 39495916 ]
- Camp-dependent protein kinase catalytic subunit alpha (40 kDa, Protein from Bos taurus)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dephosphorylated cftr in 1:1 complex with pka-c (position i) (210 kDa, Complex from Bos taurus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Dephosphorylated (E1371Q)CFTR in complex with PKA-C
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9dw8
Q-score: 0.492
Fiedorczuk K, Iordanov I, Mihalyi C, Szollosi A, Csanady L, Chen J
PNAS (2024) 121 pp. e2409049121-e2409049121 [ DOI: doi:10.1073/pnas.2409049121 Pubmed: 39495916 ]
- N-(2-phenylethyl)adenosine 5'-(tetrahydrogen triphosphate) (611 Da, Ligand)
- Camp-dependent protein kinase catalytic subunit alpha (40 kDa, Protein from Bos taurus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Dephosphorylated (e1371q)cftr in complex with pka-c (210 kDa, Complex from Bos taurus)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8eig
Q-score: 0.506
Science (2022) 378 pp. 284-290 [ DOI: doi:10.1126/science.ade2216 Pubmed: 36264792 ]
- The complex of phosphorylated human delta f508 cystic fibrosis transmembrane conductance regulator (cftr) with elexacaftor (vx-445) and atp/mg (Complex from Homo sapiens)
- (6p)-n-(1,3-dimethyl-1h-pyrazole-4-sulfonyl)-6-[3-(3,3,3-trifluoro-2,2-dimethylpropoxy)-1h-pyrazol-1-yl]-2-[(4s)-2,2,4-trimethylpyrrolidin-1-yl]pyridine-3-carboxamide (597 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8eiq
Q-score: 0.583
Science (2022) 378 pp. 284-290 [ DOI: doi:10.1126/science.ade2216 Pubmed: 36264792 ]
- (6p)-n-(1,3-dimethyl-1h-pyrazole-4-sulfonyl)-6-[3-(3,3,3-trifluoro-2,2-dimethylpropoxy)-1h-pyrazol-1-yl]-2-[(4s)-2,2,4-trimethylpyrrolidin-1-yl]pyridine-3-carboxamide (597 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- N-(2,4-di-tert-butyl-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide (392 Da, Ligand)
- Tezacaftor (520 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- The complex of phosphorylated human delta f508 cystic fibrosis transmembrane conductance regulator (cftr) with trikafta [elexacaftor (vx-445), tezacaftor (vx-661), ivacaftor (vx-770)] and atp/mg (168 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Dodecane (170 Da, Ligand)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7sv7
Q-score: 0.463
Cell (2022) 185 pp. 158-168.e11 [ DOI: doi:10.1016/j.cell.2021.12.009 Pubmed: 34995514 ]
- Tetradecane (198 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- The complex of phosphorylated human cystic fibrosis transmembrane conductance regulator (cftr) with atp/mg and tezacaftor (vx-661) (Complex from Homo sapiens)
- Tezacaftor (520 Da, Ligand)
- Nonane (128 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Heptadecane (240 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Dodecane (170 Da, Ligand)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7svr
Q-score: 0.42
Cell (2022) 185 pp. 158-168.e11 [ DOI: doi:10.1016/j.cell.2021.12.009 Pubmed: 34995514 ]
- Lumacaftor (452 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- The complex of dephosphorylated human cystic fibrosis transmembrane conductance regulator (cftr) and lumacaftor (vx-809) (Complex from Homo sapiens)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
CI Membrane Arm focused refinement from Ovine respiratory SC I+III2
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6q9b
Q-score: 0.403
Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA
Mol Cell (2019) 75 pp. 1131-1146.e6 [ Pubmed: 31492636 DOI: doi:10.1016/j.molcel.2019.07.022 ]
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit a3 (9 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Ovis aries)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (5 kDa, Protein from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase core subunit s2,nadh:ubiquinone oxidoreductase core subunit s2 (49 kDa, Protein from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from Ovis aries)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b3 (11 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit s5 (12 kDa, Protein from Ovis aries)
- Acyl carrier protein (10 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b7 (16 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b5 (16 kDa, Protein from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b2 (8 kDa, Protein from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Ovis aries)
- Ovine mitochondrial sc i+iii2 (1 MDa, Complex from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Ovis aries)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (14 kDa, Protein from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b9 (21 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b10 (20 kDa, Protein from Ovis aries)
- Cardiolipin (1 kDa, Ligand)
CI Peripheral Arm focused refinement from Ovine respiratory SC I+III2
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6q9d
Q-score: 0.424
Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA
Mol Cell (2019) 75 pp. 1131-1146.e6 [ Pubmed: 31492636 DOI: doi:10.1016/j.molcel.2019.07.022 ]
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from Ovis aries)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit v3 (8 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit a9 (38 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (48 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase core subunit s2,nadh:ubiquinone oxidoreductase core subunit s2 (49 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit s4 (15 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from Ovis aries)
- Acyl carrier protein (10 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase core subunit s7 (20 kDa, Protein from Ovis aries)
- Nadh:ubiquinone oxidoreductase core subunit s8 (20 kDa, Protein from Ovis aries)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Ovine mitochondrial sc i+iii2 (1 MDa, Complex from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit a6 (14 kDa, Protein from Ovis aries)
- Zinc ion (65 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase core subunit s3 (26 kDa, Protein from Ovis aries)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh:ubiquinone oxidoreductase core subunit s1 (77 kDa, Protein from Ovis aries)
Complex III2 focused refinement from Ovine respiratory supercomplex I+III2
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6q9e
Q-score: 0.413
Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA
Mol Cell (2019) 75 pp. 1131-1146.e6 [ Pubmed: 31492636 DOI: doi:10.1016/j.molcel.2019.07.022 ]
- Ubiquinol-cytochrome c reductase complex iii subunit vii (9 kDa, Protein from Ovis aries)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (21 kDa, Protein from Ovis aries)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (2 kDa, Protein from Ovis aries)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ubiquinol-cytochrome c reductase, complex iii subunit x (7 kDa, Protein from Ovis aries)
- Ubiquinol-cytochrome c reductase core protein 1 (49 kDa, Protein from Ovis aries)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Ovis aries)
- Cardiolipin (1 kDa, Ligand)
- Cytochrome b (42 kDa, Protein from Ovis aries)
- Ubiquinone-10 (863 Da, Ligand)
- Cytochrome c1 (27 kDa, Protein from Ovis aries)
- Cytochrome b-c1 complex subunit 6 (9 kDa, Protein from Ovis aries)
- Ovine mitochondrial sc i+iii2 (1 MDa, Complex from Ovis aries)
- Heme c (618 Da, Ligand)
- Ubiquinol-cytochrome c reductase core protein 2 (46 kDa, Protein from Ovis aries)
Entire ovine respiratory complex I, Combined Map
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5lnk
Q-score: 0.408
Fiedorczuk K, Letts JA, Degliesposti G, Kaszuba K, Skehel M, Sazanov LA
Nature (2016) 538 pp. 406-410 [ Pubmed: 27595392 DOI: doi:10.1038/nature19794 ]
- Mitochondrial complex i, 42 kda subunit (36 kDa, Protein from Sheep)
- Mitochondrial complex i, b22 subunit (21 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, 39 kda subunit (38 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, 15 kda subunit (12 kDa, Protein from Sheep)
- 4'-phosphopantetheine (358 Da, Ligand)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Mitochondrial complex i, 51 kda subunit (48 kDa, Protein from Sheep)
- Mitochondrial complex i, nd4 subunit (52 kDa, Protein from Sheep)
- Mitochondrial complex i, sgdh subunit (16 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Mitochondrial complex i, nd6 subunit (19 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5b subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i (970 kDa, Complex from Ovis aries)
- Mitochondrial complex i, nd4l subunit (10 kDa, Protein from Sheep)
- Mitochondrial complex i, 49 kda subunit (47 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (6 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, 75 kda subunit (77 kDa, Protein from Sheep)
- Mitochondrial complex i, esss subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, 13 kda subunit (10 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (5 kDa, Protein from Sheep)
- Mitochondrial complex i, nd5 subunit (67 kDa, Protein from Sheep)
- Mitochondrial complex i, 18 kda subunit (15 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Mitochondrial complex i, pgiv subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, 30 kda subunit (26 kDa, Protein from Sheep)
- Mitochondrial complex i, 10 kda subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, b18 subunit (16 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Acyl carrier protein (10 kDa, Protein from Sheep)
- Mitochondrial complex i, b8 subunit (10 kDa, Protein from Sheep)
- Mitochondrial complex i, nd3 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, b17.2 subunit (17 kDa, Protein from Sheep)
- Mitochondrial complex i, aggg subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, nd2 subunit (39 kDa, Protein from Sheep)
- Mitochondrial complex i, b12 subunit (11 kDa, Protein from Sheep)
- Mitochondrial complex i, b15 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b9 subunit (9 kDa, Protein from Sheep)
- Mitochondrial complex i, b14 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.7 subunit (10 kDa, Protein from Sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Mitochondrial complex i, tyky subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, 24 kda subunit (23 kDa, Protein from Sheep)
- Mitochondrial complex i, nd1 subunit (35 kDa, Protein from Sheep)