Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ Pubmed: 36782050 DOI: 10.1038/s41594-022-00918-0 ]
Image sets:
KS-AT di-domain of mycobacterial Pks13 with endogenous KS ligand bound
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 7uk4
Q-score: 0.628
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
- Water (18 Da, Ligand)
- Unknown ligand (312 Da, Ligand)
- Ketosynthase domain (Complex from Mycolicibacterium smegmatis MC2 155)
- Acyl transferase domain (Complex from Mycolicibacterium smegmatis MC2 155)
- Polyketide synthase pks13 (198 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mycobacterial polyketide synthase 13 (Complex from Mycolicibacterium smegmatis MC2 155)
ACP1-KS-AT domains of mycobacterial Pks13
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8cuy
Q-score: 0.574
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
KS-AT domains of mycobacterial Pks13 with inward AT conformation
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8cuz
Q-score: 0.522
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
KS-AT domains of mycobacterial Pks13 with outward AT conformation
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8cv0
Q-score: 0.508
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
ACP1-KS-AT domains of mycobacterial Pks13
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8cv1
Q-score: 0.543
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
- Water (18 Da, Ligand)
- Unknown ligand (312 Da, Ligand)
- Polyketide synthase pks13 (194 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mycobacterial polyketide synthase 13 (Complex from Mycolicibacterium smegmatis MC2 155)