human MutSalpha (MSH2/MSH6) binding to DNA with a GT mismatch
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8ag6
Q-score: 0.542
Bruekner SR, Pieters W, Fish A, Liaci AM, Scheffers S, Rayner E, Kaldenbach D, Drost L, Dekker M, van Hees-Stuivenberg S, Delzenne-Goette E, de Konink C, Houlleberghs H, Dubbink HJ, AlSaegh A, de Wind N, Forster F, Te Riele H, Sixma TK
Nucleic Acids Res (2023) 51 pp. 1173-1188 [ Pubmed: 36715327 DOI: doi:10.1093/nar/gkad015 ]
- Mutsalpha on mismatched dna (31 kDa, Complex from Homo sapiens)
- Msh6 (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna mismatch repair protein msh6 (158 kDa, Protein from Homo sapiens)
- Dna (50-mer) (15 kDa, DNA from synthetic construct)
- Dna (50-mer) (15 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Msh2 (Complex from Homo sapiens)
- Dna containing a g/t mismatch (Complex from Homo sapiens)
human MutSalpha mutant (MSH2-V63E/MSH6) on DNA containing a GT mismatch
Bruekner SR, Pieters W, Fish A, Liaci AM, Scheffers S, Rayner E, Kaldenbach D, Drost L, Dekker M, van Hees-Stuivenberg S, Delzenne-Goette E, de Konink C, Houllleberghs H, Dubbink HJ, AlSaegh A, de Wind N, Forster F, te Riele H, Sixma TK
Nucleic Acids Res (2023) [ ]
- Msh2 v63e (Protein from Homo sapiens)
- Msh6 (Complex from Homo sapiens)
- Msh6 (Protein from Homo sapiens)
- Msh2 v63e (Complex from Homo sapiens)
- Human mutsalpha mutant (msh2-v63e/msh6) on mismatched dna (31 kDa, Complex from Homo sapiens)
- Dna (50-mer) (DNA from synthetic construct)
- Dna (50-mer) (Complex from synthetic construct)
human MutSalpha (MSH2/MSH6) on DNA containing a GT mismatch in the presence of ADP
Bruekner SR, Pieters W, Fish A, Liaci AM, Scheffers S, Rayner E, Kaldenbach D, Drost L, Dekker M, van Hees-Stuivenberg S, Delzenne-Goette E, de Konink C, Houlleberghs H, Dubbink HJ, AlSaegh A, de Wind N, Forster F, Te Riele H, Sixma TK
Nucleic Acids Res (2023) 51 pp. 1173-1188 [ Pubmed: 36715327 DOI: doi:10.1093/nar/gkad015 ]
- Msh6 (Complex from Homo sapiens)
- Msh6 (Protein from Homo sapiens)
- Mutsalpha on mismatched dna in the presence of adp (31 kDa, Complex from Homo sapiens)
- Dna (50-mer) (DNA from synthetic construct)
- Msh2 (Complex from Homo sapiens)
- Dna (50-mer) (Complex from synthetic construct)
- Msh2 (Protein from Homo sapiens)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7ai5
Q-score: 0.239
Fernandez-Leiro R, Bhairosing-Kok D, Kunetsky V, Laffeber C, Winterwerp HH, Groothuizen F, Fish A, Lebbink JHG, Friedhoff P, Sixma TK, Lamers MH
Nat Struct Mol Biol (2021) 28 pp. 373-381 [ DOI: doi:10.1038/s41594-021-00577-7 Pubmed: 33820992 ]
- Dna (5'-d(p*cp*tp*ap*tp*ap*gp*gp*gp*cp*gp*ap*ap*tp*tp*gp*gp*gp*tp*ap*cp*cp*g)-3') (6 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Muts loaded on matched dna in the presence of atp (190 kDa, Complex)
- Dna (Complex from synthetic construct)
- Dna mismatch repair protein muts (95 kDa, Protein from Escherichia coli)
- Dna (5'-d(p*cp*gp*gp*tp*ap*cp*cp*cp*ap*ap*tp*tp*cp*gp*cp*cp*cp*tp*ap*tp*ap*g)-3') (6 kDa, DNA from synthetic construct)
- Dna mismatch repair protein muts (Complex from Escherichia coli)
Resolution: 6.9 Å
EM Method: Single-particle
Fitted PDBs: 7ai6
Q-score: 0.144
Fernandez-Leiro R, Bhairosing-Kok D, Kunetsky V, Laffeber C, Winterwerp HH, Groothuizen F, Fish A, Lebbink JHG, Friedhoff P, Sixma TK, Lamers MH
Nat Struct Mol Biol (2021) 28 pp. 373-381 [ DOI: doi:10.1038/s41594-021-00577-7 Pubmed: 33820992 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Muts loaded on dna substrate with one central mismatched basepair in the presence of amp-pnp (190 kDa, Complex)
- Dna (25-mer) (7 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Dna mismatch repair protein muts (Complex from Escherichia coli (strain K12))
- Dna (25-mer) (7 kDa, DNA from synthetic construct)
- Dna mismatch repair protein muts (95 kDa, Protein from Escherichia coli (strain K12))
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7ai7
Q-score: 0.336
Fernandez-Leiro R, Bhairosing-Kok D, Kunetsky V, Laffeber C, Winterwerp HH, Groothuizen F, Fish A, Lebbink JHG, Friedhoff P, Sixma TK, Lamers MH
Nat Struct Mol Biol (2021) 28 pp. 373-381 [ DOI: doi:10.1038/s41594-021-00577-7 Pubmed: 33820992 ]
- Dna mismatch repair protein muts (95 kDa, Protein from Escherichia coli (strain K12))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Muts loaded on matched dna in the presence of atp (190 kDa, Complex)
- Dna (5'-d(p*gp*ap*tp*cp*cp*tp*ap*ap*cp*tp*ap*ap*g)-3') (4 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Dna mismatch repair protein muts (Complex from Escherichia coli (strain K12))
- Dna (5'-d(p*cp*tp*tp*ap*gp*cp*tp*tp*ap*gp*gp*ap*tp*c)-3') (4 kDa, DNA from synthetic construct)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 7aib
Q-score: 0.198
Fernandez-Leiro R, Bhairosing-Kok D, Kunetsky V, Laffeber C, Winterwerp HH, Groothuizen F, Fish A, Lebbink JHG, Friedhoff P, Sixma TK, Lamers MH
Nat Struct Mol Biol (2021) 28 pp. 373-381 [ DOI: doi:10.1038/s41594-021-00577-7 Pubmed: 33820992 ]
- Dna mismatch repair protein mutl (39 kDa, Protein from Escherichia coli (strain K12))
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Dna (Complex from synthetic construct)
- Muts loaded on dna substrate with one central mismatched basepair in the presence of amp-pnp (280 kDa, Complex)
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Dna mismatch repair protein muts and dna mismatch repair endonuclease mutl (Complex from Escherichia coli (strain K12))
- Dna mismatch repair protein muts (95 kDa, Protein from Escherichia coli (strain K12))
MutS-MutL in clamp state (kinked clamp domain)
Resolution: 5.0 Å
EM Method: Single-particle
Fitted PDBs: 7aic
Q-score: 0.158
Fernandez-Leiro R, Bhairosing-Kok D, Kunetsky V, Laffeber C, Winterwerp HH, Groothuizen F, Fish A, Lebbink JHG, Friedhoff P, Sixma TK, Lamers MH
Nat Struct Mol Biol (2021) 28 pp. 373-381 [ DOI: doi:10.1038/s41594-021-00577-7 Pubmed: 33820992 ]
- Dna mismatch repair protein mutl (39 kDa, Protein from Escherichia coli (strain K12))
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Dna (Complex from synthetic construct)
- Muts loaded on dna substrate with one central mismatched basepair in the presence of amp-pnp (280 kDa, Complex)
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Dna mismatch repair protein muts and dna mismatch repair endonuclease mutl (Complex from Escherichia coli (strain K12))
- Dna mismatch repair protein muts (95 kDa, Protein from Escherichia coli (strain K12))