Cryo-EM structure of a human ATP11C-CDC50A flippase in E1-AMPPCP state
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7bsp
Q-score: 0.477
Nakanishi H, Nishizawa T, Segawa K, Nureki O, Fujiyoshi Y, Nagata S, Abe K
Cell Rep (2020) 32 pp. 108208-108208 [ Pubmed: 32997992 DOI: doi:10.1016/j.celrep.2020.108208 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cdc50a (40 kDa, Protein from Homo sapiens)
- Atp11c (124 kDa, Protein from Homo sapiens)
- Human atp11c-cdc50a flippase (180 kDa, Complex from Homo sapiens)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
Cryo-EM structure of a human ATP11C-CDC50A flippase in E1AlF-ADP state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7bsq
Q-score: 0.567
Nakanishi H, Nishizawa T, Segawa K, Nureki O, Fujiyoshi Y, Nagata S, Abe K
Cell Rep (2020) 32 pp. 108208-108208 [ Pubmed: 32997992 DOI: doi:10.1016/j.celrep.2020.108208 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cdc50a (40 kDa, Protein from Homo sapiens)
- Atp11c (124 kDa, Protein from Homo sapiens)
- Human atp11c-cdc50a flippase (180 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Cryo-EM structure of a human ATP11C-CDC50A flippase in E1AlF state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7bss
Q-score: 0.539
Nakanishi H, Nishizawa T, Segawa K, Nureki O, Fujiyoshi Y, Nagata S, Abe K
Cell Rep (2020) 32 pp. 108208-108208 [ Pubmed: 32997992 DOI: doi:10.1016/j.celrep.2020.108208 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cdc50a (40 kDa, Protein from Homo sapiens)
- Alpha-d-mannopyranose (180 Da, Ligand)
- Atp11c (124 kDa, Protein from Homo sapiens)
- Human atp11c-cdc50a flippase (180 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Cryo-EM structure of a human ATP11C-CDC50A flippase in PtdSer-bound E2BeF state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7bsu
Q-score: 0.572
Nakanishi H, Nishizawa T, Segawa K, Nureki O, Fujiyoshi Y, Nagata S, Abe K
Cell Rep (2020) 32 pp. 108208-108208 [ Pubmed: 32997992 DOI: doi:10.1016/j.celrep.2020.108208 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Human atp11c-cdc50a flippase (180 kDa, Complex from Homo sapiens)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- Atp11c (124 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 1-deoxy-alpha-d-mannopyranose (164 Da, Ligand)
Cryo-EM structure of a human ATP11C-CDC50A flippase in PtdSer-occluded E2-AlF state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7bsv
Q-score: 0.585
Nakanishi H, Nishizawa T, Segawa K, Nureki O, Fujiyoshi Y, Nagata S, Abe K
Cell Rep (2020) 32 pp. 108208-108208 [ Pubmed: 32997992 DOI: doi:10.1016/j.celrep.2020.108208 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cdc50a (40 kDa, Protein from Homo sapiens)
- Atp11c (124 kDa, Protein from Homo sapiens)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Human atp11c-cdc50a flippase (180 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Cryo-EM structure of a human ATP11C-CDC50A flippase in PtdEtn-occluded E2-AlF state
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7bsw
Q-score: 0.475
Nakanishi H, Nishizawa T, Segawa K, Nureki O, Fujiyoshi Y, Nagata S, Abe K
Cell Rep (2020) 32 pp. 108208-108208 [ Pubmed: 32997992 DOI: doi:10.1016/j.celrep.2020.108208 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cdc50a (40 kDa, Protein from Homo sapiens)
- Alpha-d-mannopyranose (180 Da, Ligand)
- Atp11c (124 kDa, Protein from Homo sapiens)
- Human atp11c-cdc50a flippase (180 kDa, Complex from Homo sapiens)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (749 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Gastric H,K-ATPase complexed with aluminum fluoride
Resolution: 6.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 3ixz
Q-score: 0.106
Abe K, Tani K, Nishizawa T, Fujiyoshi Y
EMBO J (2009) 28 pp. 1637-1643 [ DOI: doi:10.1038/emboj.2009.102 Pubmed: 19387495 ]
- Gastric h,k-atpase (150 kDa, Sample)
- Potassium-transporting atpase (150 kDa, Protein from Sus scrofa)