Structure of the human nuclear cap-binding complex bound to NCBP3(560-620) and cap-analogue m7GpppG
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8by6
Q-score: 0.483
Dubiez E, Pellegrini E, Finderup Brask M, Garland W, Foucher AE, Huard K, Heick Jensen T, Cusack S, Kadlec J
Cell Rep (2024) 43 pp. 113639-113639 [ DOI: doi:10.1016/j.celrep.2023.113639 Pubmed: 38175753 ]
- Nuclear cap-binding protein subunit 3 (6 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 1 (89 kDa, Protein from Homo sapiens)
- Ternary complex of cbp80, cbp20 and ncbp3 with bound m7gpppg (Complex from Homo sapiens)
- 7-methyl-guanosine-5'-triphosphate-5'-guanosine (803 Da, Ligand)
Structure of the human nuclear cap-binding complex bound to ARS2[147-871] and m7GTP
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8pmp
Q-score: 0.318
Dubiez E, Pellegrini E, Finderup Brask M, Garland W, Foucher AE, Huard K, Heick Jensen T, Cusack S, Kadlec J
Cell Rep (2024) 43 pp. 113639-113639 [ DOI: doi:10.1016/j.celrep.2023.113639 Pubmed: 38175753 ]
- Nuclear cap-binding protein subunit 1 (89 kDa, Protein from Homo sapiens)
- Structure of the human nuclear cap-binding complex bound to ars2[147-871] and m7gtp (Complex from Homo sapiens)
- 7n-methyl-8-hydroguanosine-5'-triphosphate (539 Da, Ligand)
- Serrate rna effector molecule homolog (83 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
Structure of the human nuclear cap-binding complex bound to PHAX and m7G-capped RNA
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 8pnt
Q-score: 0.419
Dubiez E, Pellegrini E, Finderup Brask M, Garland W, Foucher AE, Huard K, Heick Jensen T, Cusack S, Kadlec J
Cell Rep (2024) 43 pp. 113639-113639 [ DOI: doi:10.1016/j.celrep.2023.113639 Pubmed: 38175753 ]
- Phosphorylated adapter rna export protein (44 kDa, Protein from Homo sapiens)
- 7n-methyl-8-hydroguanosine-5'-triphosphate (539 Da, Ligand)
- Structure of the human nuclear cap-binding complex bound to phax and m7g-capped rna (Complex from Homo sapiens)
- Nuclear cap-binding protein subunit 1 (89 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
Human NEXT dimer - overall reconstruction of the core complex
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7z4y
Q-score: 0.257
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Mol Cell (2022) 82 pp. 2505- [ Pubmed: 35688157 DOI: doi:10.1016/j.molcel.2022.04.011 ]
Human NEXT dimer - focused reconstruction of the dimerization module
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7z4z
Q-score: 0.392
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Mol Cell (2022) 82 pp. 2505- [ Pubmed: 35688157 DOI: doi:10.1016/j.molcel.2022.04.011 ]
Human NEXT dimer - focused reconstruction of the single MTR4
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7z52
Q-score: 0.512
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Mol Cell (2022) 82 pp. 2505- [ Pubmed: 35688157 DOI: doi:10.1016/j.molcel.2022.04.011 ]
- Human next dimer - focused reconstruction of the single mtr4 (Complex from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Exosome rna helicase mtr4 (118 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*up*up*up*up*u)-3') (1 kDa, RNA from synthetic construct)
- Zinc finger cchc domain-containing protein 8 (78 kDa, Protein from Homo sapiens)
Human NEXT dimer - single protomer
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Mol Cell (2022) 82 pp. 2505-2518.e7 [ Pubmed: 35688157 DOI: doi:10.1016/j.molcel.2022.04.011 ]
- Rna 5prime hairpin u60 (Complex from synthetic construct)
- Zinc finger cchc domain-containing protein 8 (Protein from Homo sapiens)
- Rna 5prime hairpin u60 (RNA from synthetic construct)
- Rna-binding protein 7 (Protein from Homo sapiens)
- Human next dimer - single protomer (Complex)
- Exosome rna helicase mtr4 (Protein from Homo sapiens)
- Human next dimer - single protomer (Complex from Homo sapiens)
Human NEXT dimer in complex with the nuclear RNA exosome
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Mol Cell (2022) 82 pp. 2505-2518.e7 [ Pubmed: 35688157 DOI: doi:10.1016/j.molcel.2022.04.011 ]
- Zinc finger cchc domain-containing protein 8 (Protein from Homo sapiens)
- Nuclear nucleic acid-binding protein c1d (Protein from Homo sapiens)
- Rna 5 prime hairpin u60 (RNA from synthetic construct)
- Exosome complex component rrp46 (Protein from Homo sapiens)
- Human next dimer in complex with the nuclear rna exosome (Complex from Homo sapiens)
- Human next dimer in complex with the nuclear rna exosome (Complex)
- Exosome rna helicase mtr4 (Protein from Homo sapiens)
- M-phase phosphoprotein 6 (Protein from Homo sapiens)
- Exosome complex component mtr3 (Protein from Homo sapiens)
- Rna 5 prime hairpin u60 (Complex from synthetic construct)
- Exosome complex component rrp42 (Protein from Homo sapiens)
- Exosome complex component rrp45 (Protein from Homo sapiens)
- Exosome complex component rrp40 (Protein from Homo sapiens)
- Exosome complex component csl4 (Protein from Homo sapiens)
- Exosome component 10 (Protein from Homo sapiens)
- Rna-binding protein 7 (Protein from Homo sapiens)
- Exosome complex component rrp41 (Protein from Homo sapiens)
- Exosome complex exonuclease rrp44 (Protein from Homo sapiens)
- Exosome complex component rrp4 (Protein from Homo sapiens)