Xenopus laevis hyaluronan synthase 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8smm
Q-score: 0.504
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Fab15 light chain (23 kDa, Protein from Homo sapiens)
- Fab15 heavy chain (24 kDa, Protein from Homo sapiens)
- Hyaluronan synthase 1 (70 kDa, Protein from Xenopus laevis)
- Xenopus laevis hyaluronan synthase 1 in complex with a fab molecule (118 kDa, Complex from Xenopus laevis)
Xenopus laevis hyaluronan synthase 1, nascent HA polymer bound state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8smn
Q-score: 0.557
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Water (18 Da, Ligand)
- Fab15 light chain (23 kDa, Protein from Homo sapiens)
- Fab15 heavy chain (24 kDa, Protein from Homo sapiens)
- Hyaluronan synthase 1 (70 kDa, Protein from Xenopus laevis)
- Xenopus laevis hyaluronan synthase 1 in complex with a fab molecule, nascent ha polymer bound state (118 kDa, Complex from Xenopus laevis)
Xenopus laevis hyaluronan synthase 1, UDP-bound, gating loop inserted state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8smp
Q-score: 0.511
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Uridine-5'-diphosphate (404 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Fab15 light chain (23 kDa, Protein from Homo sapiens)
- Fab15 heavy chain (24 kDa, Protein from Homo sapiens)
- Hyaluronan synthase 1 (70 kDa, Protein from Xenopus laevis)
- Xenopus laevis hyaluronan synthase 1, udp-bound, gating loop inserted state (118 kDa, Complex from Xenopus laevis)
Chlorella virus Hyaluronan Synthase bound to GlcA extended GlcNAc primer
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8snc
Q-score: 0.509
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Ternary complex of chlorella virus hyaluronan synthase bound to nanobodies 872 and 886 (95 MDa, Complex from Paramecium bursaria Chlorella virus 1)
- Water (18 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Nanobody 872 (14 kDa, Protein from Lama glama)
Chlorella virus Hyaluronan Synthase bound to GlcNAc primer and UDP-GlcA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8snd
Q-score: 0.512
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Uridine-5'-diphosphate-glucuronic acid (580 Da, Ligand)
- Water (18 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Nanobody 872 (14 kDa, Protein from Lama glama)
- Ternary complex of chlorella virus hyaluronan synthase bound to nanobodies 872 and 881 (97 MDa, Complex from Paramecium bursaria Chlorella virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Chlorella virus Hyaluronan Synthase bound to GlcA extended GlcNAc primer and UDP
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8sne
Q-score: 0.546
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Uridine-5'-diphosphate (404 Da, Ligand)
- Ternary complex of chlorella virus hyaluronan synthase bound to nanobodies 872 and 886 (95 MDa, Complex from Paramecium bursaria Chlorella virus 1)
- Manganese (ii) ion (54 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Nanobody 886 (14 kDa, Protein from Lama glama)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nanobody 872 (14 kDa, Protein from Lama glama)
Cryo-EM structure of FLVCR2 in the inward-facing state with choline bound
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8vzn
Q-score: 0.583
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ Pubmed: 38693257 DOI: doi:10.1038/s41586-024-07326-y ]
- Flvcr2 in the inward-facing state complexed with choline and fab flv9 (110 kDa, Complex from Mus musculus)
- Fab flv9 light chain (23 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
- Fab flv9 heavy chain (25 kDa, Protein from synthetic construct)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
Cryo-EM structure of FLVCR2 in the outward-facing state with choline bound
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8vzo
Q-score: 0.637
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ Pubmed: 38693257 DOI: doi:10.1038/s41586-024-07326-y ]
- Fab flv23 heavy chain (25 kDa, Protein from synthetic construct)
- Flvcr2 in the outward-facing state complexed with choline and fab flv23 (110 kDa, Complex from Mus musculus)
- Fab flv23 light chain (23 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)