CryoEM Structure of the, UND-PP bound, WaaL O-Antigen Ligase
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ Pubmed: 35388216 DOI: 10.1038/s41586-022-04555-x ]
Image sets:
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7tpg
Q-score: 0.497
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ Pubmed: 35388216 DOI: doi:10.1038/s41586-022-04555-x ]
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Geranyl diphosphate (314 Da, Ligand)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
- Ligand bound cmwaal with fab fragment bound (Complex)
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its apo state
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7tpj
Q-score: 0.431
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ Pubmed: 35388216 DOI: doi:10.1038/s41586-022-04555-x ]
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Apo cmwaal with fab fragment bound (Complex)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
Tan YZ, Rodrigues J, Keener JE, Zheng RB, Brunton R, Kloss B, Giacometti SI, Rosário AL, Zhang L, Niederweis M, Clarke OB, Lowary TL, Marty MT, Archer M, Potter CS, Carragher B, Mancia F, Tan YZ, Rodrigues J, Keener JE, Zheng RB, Brunton R, Kloss B, Giacometti SI, Rosário AL, Zhang L, Niederweis M, Clarke OB, Lowary TL, Marty MT, Archer M, Potter CS, Carragher B, Mancia F
Nat Commun (2020) 11 pp. 3396-3396 [ DOI: 10.1038/s41467-020-17202-8 Pubmed: 32636380 ]
Image sets:
- Aligned and dose-weighted micrographs from Falcon III Detector
- Unaligned and compressed multiframe movies from Falcon III Detector
- Unaligned and compressed multiframe movies from K2 Summit Detector
- Final Particle Stack with Refined Euler Angles and Shifts
- Aligned and dose-weighted micrographs from K2 Summit Detector
Single-Particle Cryo-EM Structure of Arabinosyltransferase EmbB from Mycobacterium smegmatis
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6x0o
Q-score: 0.508
Tan YZ, Rodrigues J, Keener JE, Zheng RB, Brunton R, Kloss B, Giacometti SI, Rosario AL, Zhang L, Niederweis M, Clarke OB, Lowary TL, Marty MT, Archer M, Potter CS, Carragher B, Mancia F
Nat Commun (2020) 11 pp. 3396-3396 [ DOI: doi:10.1038/s41467-020-17202-8 Pubmed: 32636380 ]
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Integral membrane indolylacetylinositol arabinosyltransferase embb (118 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Integral membrane indolylacetylinositol arabinosyltransferase embb (119 kDa, Cellular component from Mycolicibacterium smegmatis)
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol. Cell (2020) [ DOI: 10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
Image sets:
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Wild-type
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol. Cell (2020) [ DOI: 10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
Image sets:
Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6w98
Q-score: 0.535
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
- Wild-type mycobacterial arabinofuranosyltransferase aftd complexed with acyl carrier protein (50 kDa, Cellular component)
- 4'-phosphopantetheine (358 Da, Ligand)
- F5/8 type c domain-containing protein (149 kDa, Protein from Mycobacteroides abscessus subsp. abscessus)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli (strain K12))
- F5/8 type c domain-containing protein (Complex from Mycobacteroides abscessus subsp. abscessus)
- Acyl carrier protein (Cellular component from Escherichia coli (strain K12))
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6wbx
Q-score: 0.518
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6wby
Q-score: 0.533
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]