Cryo-EM structure of the inner MKLN1 dimer from an autoinhibited MKLN1 tetramer
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8ttq
Q-score: 0.398
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ DOI: doi:10.1038/s41467-024-52009-x Pubmed: 39215025 ]
Cryo-EM structure of an autoinhibited MKLN1 tetramer
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ DOI: doi:10.1038/s41467-024-52009-x Pubmed: 39215025 ]
Cryo-EM structure of CTLH-MKLN1-FAM72A in complex with UNG2
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ DOI: doi:10.1038/s41467-024-52009-x Pubmed: 39215025 ]
Cryo-EM structure of a FAM72A-MKLN1-RANBP9-TWA1 complex
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ DOI: doi:10.1038/s41467-024-52009-x Pubmed: 39215025 ]
CryoEM structure of the HisRS-like domain of human GCN2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8t7t
Q-score: 0.45
Yin JZ, Keszei AFA, Houliston S, Filandr F, Beenstock J, Daou S, Kitaygorodsky J, Schriemer DC, Mazhab-Jafari MT, Gingras AC, Sicheri F
Structure (2024) 32 pp. 795-811.e6 [ DOI: doi:10.1016/j.str.2024.02.021 Pubmed: 38531363 ]
- Eif-2-alpha kinase gcn2 (187 kDa, Protein from Homo sapiens)
- Dimeric hisrs-like domain of human gcn2 (Complex from Homo sapiens)
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: 10.26508/lsa.202201527 ]
Image sets:
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Structure of porcine V-ATPase with mEAK7 and SidK, Rotary state 2
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7u8o
Q-score: 0.371
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- Tldc protein meak-7 (50 kDa, Protein from Sus scrofa)
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
- V-atpase in complex with recombinant sidk and recombinant meak-7 (Complex from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 1
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7u8p
Q-score: 0.334
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Porcine kidney v-atpase with sidk, rotary state 1 (Complex from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7u8q
Q-score: 0.247
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Porcine kidney v-atpase with sidk, rotary state 2 (Complex from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 3
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7u8r
Q-score: 0.301
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Porcine kidney v-atpase with sidk, rotary state 3 (Complex from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)