C. elegans TMC-2 complex, focused refinement of dimer interface
Clark S, Jeong H, Goehring A, Posert R, Gouaux E
To Be Published
- Transmembrane inner ear protein tmie (Protein from Caenorhabditis elegans)
- Calcium and integrin binding protein calm-1 (Protein from Caenorhabditis elegans)
- C. elegans tmc-2 complex (Complex from Caenorhabditis elegans)
- Transmembrane channel-like protein tmc-2 (Protein from Caenorhabditis elegans)
Structure of the C. elegans TMC-2 complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8tkp
Q-score: 0.258
Clark S, Jeong H, Posert R, Goehring A, Gouaux E
PNAS (2024) 121 pp. e2314096121-e2314096121 [ Pubmed: 38354260 DOI: doi:10.1073/pnas.2314096121 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Transmembrane channel-like protein 2 (136 kDa, Protein from Caenorhabditis elegans)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- C. elegans tmc-2 complex (Complex from Caenorhabditis elegans)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Docosane (310 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
Structure of Expanded C. elegans TMC-1 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7usw
Q-score: 0.508
Jeong H, Clark S, Goehring A, Dehghani-Ghahnaviyeh S, Rasouli A, Tajkhorshid E, Gouaux E
Nature (2022) 610 pp. 796-803 [ Pubmed: 36224384 DOI: doi:10.1038/s41586-022-05314-8 ]
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transmembrane channel-like protein 1 (147 kDa, Protein from Caenorhabditis elegans)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Undecan-1-ol (172 Da, Ligand)
- Native tmc-1 complex (430 kDa, Complex from Caenorhabditis elegans)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Cholesterol (386 Da, Ligand)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
- Dodecane (170 Da, Ligand)
Structure of Contracted C. elegans TMC-1 complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7usx
Q-score: 0.48
Jeong H, Clark S, Goehring A, Dehghani-Ghahnaviyeh S, Rasouli A, Tajkhorshid E, Gouaux E
Nature (2022) 610 pp. 796-803 [ Pubmed: 36224384 DOI: doi:10.1038/s41586-022-05314-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transmembrane channel-like protein 1 (147 kDa, Protein from Caenorhabditis elegans)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Native tmc-1 complex (430 kDa, Complex from Caenorhabditis elegans)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Cholesterol (386 Da, Ligand)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
- Dodecane (170 Da, Ligand)
Structure of C. elegans TMC-1 complex with ARRD-6
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 7usy
Q-score: 0.355
Jeong H, Clark S, Goehring A, Dehghani-Ghahnaviyeh S, Rasouli A, Tajkhorshid E, Gouaux E
Nature (2022) 610 pp. 796-803 [ Pubmed: 36224384 DOI: doi:10.1038/s41586-022-05314-8 ]
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transmembrane channel-like protein 1 (147 kDa, Protein from Caenorhabditis elegans)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Native tmc-1 complex (480 kDa, Complex from Caenorhabditis elegans)
- Arrestin domain protein (53 kDa, Protein from Caenorhabditis elegans)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
Cryo-electron tilt series of mouse stereocilia
Elferich J, Clark S, Ge J, Goehring A, Matsui A, Gouaux E
bioRxiv (2021) [ DOI: 10.1101/2021.10.01.462800 ]
Image sets:
Molecular structure and conformation of stereocilia tip-links elucidated by cryo-electron tomography
Elferich J, Clark S, Ge J, Goehring A, Matsui A, Gouaux E
bioRxiv (2021) [ DOI: 10.1101/2021.10.01.462800 ]
Image sets:
Tomogram of mPCDH15/39G7-AuNP complex
Elferich J, Clark S, Ge J, Goehring A, Matsui A, Gouaux E
eLife (2021) 10 [ Pubmed: 34964715 DOI: doi:10.7554/eLife.74512 ]
Tomogram of mouse stereocilia containing PCDH15 molecules labeled with 39G7-AuNPs
Elferich J, Clark S, Ge J, Goehring A, Matsui A, Gouaux E
eLife (2021) 10 [ Pubmed: 34964715 DOI: doi:10.7554/eLife.74512 ]
Tomogram of mouse stereocilia containing PCDH15 molecules labeled with 39G7-AuNPs
Elferich J, Clark S, Ge J, Goehring A, Matsui A, Gouaux E
eLife (2021) 10 [ Pubmed: 34964715 DOI: doi:10.7554/eLife.74512 ]