C. elegans TMC-2 complex, focused refinement of dimer interface
Clark S, Jeong H, Goehring A, Posert R, Gouaux E
To Be Published
- Transmembrane inner ear protein tmie (Protein from Caenorhabditis elegans)
- Calcium and integrin binding protein calm-1 (Protein from Caenorhabditis elegans)
- C. elegans tmc-2 complex (Complex from Caenorhabditis elegans)
- Transmembrane channel-like protein tmc-2 (Protein from Caenorhabditis elegans)
Structure of the C. elegans TMC-2 complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8tkp
Q-score: 0.258
Clark S, Jeong H, Posert R, Goehring A, Gouaux E
PNAS (2024) 121 pp. e2314096121-e2314096121 [ Pubmed: 38354260 DOI: doi:10.1073/pnas.2314096121 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Transmembrane channel-like protein 2 (136 kDa, Protein from Caenorhabditis elegans)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- C. elegans tmc-2 complex (Complex from Caenorhabditis elegans)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Docosane (310 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
Structure of Expanded C. elegans TMC-1 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7usw
Q-score: 0.508
Jeong H, Clark S, Goehring A, Dehghani-Ghahnaviyeh S, Rasouli A, Tajkhorshid E, Gouaux E
Nature (2022) 610 pp. 796-803 [ Pubmed: 36224384 DOI: doi:10.1038/s41586-022-05314-8 ]
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transmembrane channel-like protein 1 (147 kDa, Protein from Caenorhabditis elegans)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Undecan-1-ol (172 Da, Ligand)
- Native tmc-1 complex (430 kDa, Complex from Caenorhabditis elegans)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Cholesterol (386 Da, Ligand)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
- Dodecane (170 Da, Ligand)
Structure of Contracted C. elegans TMC-1 complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7usx
Q-score: 0.48
Jeong H, Clark S, Goehring A, Dehghani-Ghahnaviyeh S, Rasouli A, Tajkhorshid E, Gouaux E
Nature (2022) 610 pp. 796-803 [ Pubmed: 36224384 DOI: doi:10.1038/s41586-022-05314-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transmembrane channel-like protein 1 (147 kDa, Protein from Caenorhabditis elegans)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Native tmc-1 complex (430 kDa, Complex from Caenorhabditis elegans)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Cholesterol (386 Da, Ligand)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
- Dodecane (170 Da, Ligand)
Structure of C. elegans TMC-1 complex with ARRD-6
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 7usy
Q-score: 0.355
Jeong H, Clark S, Goehring A, Dehghani-Ghahnaviyeh S, Rasouli A, Tajkhorshid E, Gouaux E
Nature (2022) 610 pp. 796-803 [ Pubmed: 36224384 DOI: doi:10.1038/s41586-022-05314-8 ]
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transmembrane channel-like protein 1 (147 kDa, Protein from Caenorhabditis elegans)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Native tmc-1 complex (480 kDa, Complex from Caenorhabditis elegans)
- Arrestin domain protein (53 kDa, Protein from Caenorhabditis elegans)
- Calmyrin (calcium and integrin binding protein) homolog (23 kDa, Protein from Caenorhabditis elegans)
- Transmembrane inner ear expressed protein (12 kDa, Protein from Caenorhabditis elegans)
Structure of a membrane protein complex
Resolution: 11.33 Å
EM Method: Single-particle
Fitted PDBs: 6c13
Q-score: 0.074
Ge J, Elferich J, Goehring A, Zhao J, Schuck P, Gouaux E
eLife (2018) 7 [ Pubmed: 30070639 DOI: doi:10.7554/eLife.38770 ]
- Complex of membrane proteins (Complex from Mus musculus)
- Protocadherin-15 (72 kDa, Protein from Mus musculus)
cryoEM structure of membrane protein complex
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6c14
Q-score: 0.269
Ge J, Elferich J, Goehring A, Zhao J, Schuck P, Gouaux E
eLife (2018) 7 [ Pubmed: 30070639 DOI: doi:10.7554/eLife.38770 ]
- Lhfpl tetraspan subfamily member 5 protein (25 kDa, Protein from Mus musculus)
- Membrane protein complex (Complex from Mus musculus)
- Protocadherin-15 (37 kDa, Protein from Mus musculus)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5uow
Q-score: 0.206
Lu W, Du J, Goehring A, Gouaux E
Science (2017) 355 [ Pubmed: 28232581 DOI: doi:10.1126/science.aal3729 ]
- Ionotropic glutamate receptor subunit nr2b (94 kDa, Protein from Xenopus laevis)
- N-methyl-d-aspartate receptor subunit nr1-8a (91 kDa, Protein from Xenopus laevis)
- (5s,10r)-5-methyl-10,11-dihydro-5h-5,10-epiminodibenzo[a,d][7]annulene (221 Da, Ligand)
- Membrane protein (Complex from Xenopus laevis)
- N-methyl-d-aspartate receptor subunit nr2a (93 kDa, Protein from Xenopus laevis)
- Glun2b-specific fab, termed 11d1 (18 kDa, Protein from Mus musculus)
- Glutamic acid (147 Da, Ligand)
Resolution: 6.0 Å
EM Method: Single-particle
Fitted PDBs: 5up2
Q-score: 0.16
Lu W, Du J, Goehring A, Gouaux E
Science (2017) 355 [ Pubmed: 28232581 DOI: doi:10.1126/science.aal3729 ]
- Ionotropic glutamate receptor subunit nr2b (94 kDa, Protein from Xenopus laevis)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Membrane protein (Complex from Xenopus laevis)
- N-methyl-d-aspartate receptor subunit nr2a (93 kDa, Protein from Xenopus laevis)
- Glun2b-specific fab, termed 11d1 (18 kDa, Protein from Mus musculus)
- N-methyl-d-aspartate receptor subunit nr1-8a (94 kDa, Protein from Xenopus laevis)