Xenopus laevis hyaluronan synthase 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8smm
Q-score: 0.504
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Fab15 light chain (23 kDa, Protein from Homo sapiens)
- Fab15 heavy chain (24 kDa, Protein from Homo sapiens)
- Hyaluronan synthase 1 (70 kDa, Protein from Xenopus laevis)
- Xenopus laevis hyaluronan synthase 1 in complex with a fab molecule (118 kDa, Complex from Xenopus laevis)
Xenopus laevis hyaluronan synthase 1, nascent HA polymer bound state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8smn
Q-score: 0.557
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Water (18 Da, Ligand)
- Fab15 light chain (23 kDa, Protein from Homo sapiens)
- Fab15 heavy chain (24 kDa, Protein from Homo sapiens)
- Hyaluronan synthase 1 (70 kDa, Protein from Xenopus laevis)
- Xenopus laevis hyaluronan synthase 1 in complex with a fab molecule, nascent ha polymer bound state (118 kDa, Complex from Xenopus laevis)
Xenopus laevis hyaluronan synthase 1, UDP-bound, gating loop inserted state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8smp
Q-score: 0.511
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Uridine-5'-diphosphate (404 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Fab15 light chain (23 kDa, Protein from Homo sapiens)
- Fab15 heavy chain (24 kDa, Protein from Homo sapiens)
- Hyaluronan synthase 1 (70 kDa, Protein from Xenopus laevis)
- Xenopus laevis hyaluronan synthase 1, udp-bound, gating loop inserted state (118 kDa, Complex from Xenopus laevis)
Chlorella virus Hyaluronan Synthase bound to GlcA extended GlcNAc primer
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8snc
Q-score: 0.509
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Ternary complex of chlorella virus hyaluronan synthase bound to nanobodies 872 and 886 (95 MDa, Complex from Paramecium bursaria Chlorella virus 1)
- Water (18 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Nanobody 872 (14 kDa, Protein from Lama glama)
Chlorella virus Hyaluronan Synthase bound to GlcNAc primer and UDP-GlcA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8snd
Q-score: 0.512
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Uridine-5'-diphosphate-glucuronic acid (580 Da, Ligand)
- Water (18 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Nanobody 872 (14 kDa, Protein from Lama glama)
- Ternary complex of chlorella virus hyaluronan synthase bound to nanobodies 872 and 881 (97 MDa, Complex from Paramecium bursaria Chlorella virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Chlorella virus Hyaluronan Synthase bound to GlcA extended GlcNAc primer and UDP
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8sne
Q-score: 0.546
Gorniak I, Stephens Z, Erramilli SK, Gawda T, Kossiakoff AA, Zimmer J
Nat Struct Mol Biol (2025) 32 pp. 161-171 [ Pubmed: 39322765 DOI: doi:10.1038/s41594-024-01389-1 ]
- Uridine-5'-diphosphate (404 Da, Ligand)
- Ternary complex of chlorella virus hyaluronan synthase bound to nanobodies 872 and 886 (95 MDa, Complex from Paramecium bursaria Chlorella virus 1)
- Manganese (ii) ion (54 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Nanobody 886 (14 kDa, Protein from Lama glama)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nanobody 872 (14 kDa, Protein from Lama glama)
CryoEM structure of the A. aeolicus WzmWzt transporter bound to the native O antigen
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8dku
Q-score: 0.482
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ DOI: doi:10.1038/s41467-022-32597-2 Pubmed: 36064941 ]
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus VF5)
- Abc transporter (46 kDa, Protein from Aquifex aeolicus VF5)
- O antigen abc transporter (Complex from Aquifex aeolicus)
CryoEM structure of the nucleotide-free and open channel A.aeolicus WzmWzt transporter
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8dl0
Q-score: 0.307
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ DOI: doi:10.1038/s41467-022-32597-2 Pubmed: 36064941 ]
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus VF5)
- O antigen abc transporter (Complex from Aquifex aeolicus VF5)
- Abc transporter (46 kDa, Protein from Aquifex aeolicus VF5)
CryoEM structure of the A. aeolicus WzmWzt transporter bound to 3-O-methyl-D-mannose
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8dn8
Q-score: 0.394
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ DOI: doi:10.1038/s41467-022-32597-2 Pubmed: 36064941 ]
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus)
- Abc transporter (46 kDa, Protein from Aquifex aeolicus)
- 3-o-methyl-alpha-d-mannopyranose (194 Da, Ligand)
- O antigen abc transporter (Complex from Aquifex aeolicus)
CryoEM structure of the A. aeolicus WzmWzt transporter bound to the native O antigen and ADP
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8dnc
Q-score: 0.464
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ DOI: doi:10.1038/s41467-022-32597-2 Pubmed: 36064941 ]
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus)
- Magnesium ion (24 Da, Ligand)
- O antigen abc transporter (Complex from Aquifex aeolicus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Abc transporter (46 kDa, Protein from Aquifex aeolicus)