Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 8olu
Q-score: 0.649
Thomas MG, McGonagle K, Rowland P, Robinson DA, Dodd PG, Camino-Diaz I, Campbell L, Cantizani J, Castaneda P, Conn D, Craggs PD, Edwards D, Ferguson L, Fosberry A, Frame L, Goswami P, Hu X, Korczynska J, MacLean L, Martin J, Mutter N, Osuna-Cabello M, Paterson C, Pena I, Pinto EG, Pont C, Riley J, Shishikura Y, Simeons FRC, Stojanovski L, Thomas J, Wrobel K, Young RJ, Zmuda F, Zuccotto F, Read KD, Gilbert IH, Marco M, Miles TJ, Manzano P, De Rycker M
J Med Chem (2023) 66 pp. 10413-10431 [ Pubmed: 37506194 DOI: doi:10.1021/acs.jmedchem.3c00582 ]
- Proteasome alpha 1 subunit, putative (47 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit beta (23 kDa, Protein from Leishmania tarentolae)
- Proteasome beta 6 subunit, putative (37 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit beta (33 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha 7 subunit, putative (25 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit alpha type (27 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit alpha type (27 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit beta (24 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit alpha type (25 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit beta (30 kDa, Protein from Leishmania tarentolae)
- Leishmania tarentolae proteasome 20s subunit in complex with 1-benzyl-n-(3-(cyclopropylcarbamoyl)phenyl)-6-oxo-1,6-dihydropyridazine-3-carboxamide (Complex from Leishmania tarentolae)
- Proteasome subunit beta (22 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit beta (27 kDa, Protein from Leishmania tarentolae)
- ~{n}-[3-(cyclopropylcarbamoyl)phenyl]-6-oxidanylidene-1-(phenylmethyl)pyridazine-3-carboxamide (388 Da, Ligand)
- Proteasome alpha 5 subunit, putative (38 kDa, Protein from Leishmania tarentolae)
- Proteasome subunit alpha type (32 kDa, Protein from Leishmania tarentolae)
Leishmania tarentolae proteasome 20S subunit complexed with GSK3494245
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6qm7
Q-score: 0.607
Wyllie S, Brand S, Thomas M, De Rycker M, Chung CW, Pena I, Bingham RP, Bueren-Calabuig JA, Cantizani J, Cebrian D, Craggs PD, Ferguson L, Goswami P, Hobrath J, Howe J, Jeacock L, Ko EJ, Korczynska J, MacLean L, Manthri S, Martinez MS, Mata-Cantero L, Moniz S, Nuhs A, Osuna-Cabello M, Pinto E, Riley J, Robinson S, Rowland P, Simeons FRC, Shishikura Y, Spinks D, Stojanovski L, Thomas J, Thompson S, Viayna Gaza E, Wall RJ, Zuccotto F, Horn D, Ferguson MAJ, Fairlamb AH, Fiandor JM, Martin J, Gray DW, Miles TJ, Gilbert IH, Read KD, Marco M, Wyatt PG
PNAS (2019) 116 pp. 9318-9323 [ Pubmed: 30962368 DOI: doi:10.1073/pnas.1820175116 ]
- Proteasome 20s subunit (Complex from Leishmania tarentolae)
- Proteasome alpha6 chain (47 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha2 chain (25 kDa, Protein from Leishmania tarentolae)
- Proteasome beta7 chain (24 kDa, Protein from Leishmania tarentolae)
- Proteasome beta4 chain (23 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha5 chain (38 kDa, Protein from Leishmania tarentolae)
- Proteasome beta5 chain (33 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha7 chain (25 kDa, Protein from Leishmania tarentolae)
- Proteasome beta6 chain (37 kDa, Protein from Leishmania tarentolae)
- Proteasome beta2 chain (27 kDa, Protein from Leishmania tarentolae)
- ~{n}-[4-fluoranyl-3-(3-morpholin-4-ylimidazo[1,2-a]pyrimidin-7-yl)phenyl]pyrrolidine-1-carboxamide (410 Da, Ligand)
- Proteasome beta1 chain (30 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha3 chain (32 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha4 chain (27 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha1 chain (27 kDa, Protein from Leishmania tarentolae)
- Proteasome beta3 chain (22 kDa, Protein from Leishmania tarentolae)
- Water (18 Da, Ligand)
Leishmania tarentolae proteasome 20S subunit apo structure
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qm8
Q-score: 0.516
Wyllie S, Brand S, Thomas M, De Rycker M, Chung CW, Pena I, Bingham RP, Bueren-Calabuig JA, Cantizani J, Cebrian D, Craggs PD, Ferguson L, Goswami P, Hobrath J, Howe J, Jeacock L, Ko EJ, Korczynska J, MacLean L, Manthri S, Martinez MS, Mata-Cantero L, Moniz S, Nuhs A, Osuna-Cabello M, Pinto E, Riley J, Robinson S, Rowland P, Simeons FRC, Shishikura Y, Spinks D, Stojanovski L, Thomas J, Thompson S, Viayna Gaza E, Wall RJ, Zuccotto F, Horn D, Ferguson MAJ, Fairlamb AH, Fiandor JM, Martin J, Gray DW, Miles TJ, Gilbert IH, Read KD, Marco M, Wyatt PG
PNAS (2019) 116 pp. 9318-9323 [ Pubmed: 30962368 DOI: doi:10.1073/pnas.1820175116 ]
- Proteasome 20s subunit (Complex from Leishmania tarentolae)
- Proteasome alpha6 chain (47 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha2 chain (25 kDa, Protein from Leishmania tarentolae)
- Proteasome beta7 chain (24 kDa, Protein from Leishmania tarentolae)
- Proteasome beta4 chain (23 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha5 chain (38 kDa, Protein from Leishmania tarentolae)
- Proteasome beta5 chain (33 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha7 chain (25 kDa, Protein from Leishmania tarentolae)
- Proteasome beta6 chain (37 kDa, Protein from Leishmania tarentolae)
- Proteasome beta2 chain (27 kDa, Protein from Leishmania tarentolae)
- Proteasome beta1 chain (30 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha3 chain (32 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha4 chain (27 kDa, Protein from Leishmania tarentolae)
- Proteasome alpha1 chain (27 kDa, Protein from Leishmania tarentolae)
- Proteasome beta3 chain (22 kDa, Protein from Leishmania tarentolae)
Cryo-EM structure of S. cerevisiae Polymerase epsilon deltacat mutant
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6hv8
Q-score: 0.315
Goswami P, Abid Ali F, Douglas ME, Locke J, Purkiss A, Janska A, Eickhoff P, Early A, Nans A, Cheung AMC, Diffley JFX, Costa A
Nat Commun (2018) 9 pp. 5061-5061 [ Pubmed: 30498216 DOI: doi:10.1038/s41467-018-07417-1 ]
- Dna polymerase epsilon catalytic subunit a (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Cryo-em structure of s. cerevisiae polymerase epsilon deltacat (c-pol2+c-dpb2) (Complex from Saccharomyces cerevisiae)
S. cerevisiae CMG-Pol epsilon-DNA
Resolution: 4.98 Å
EM Method: Single-particle
Fitted PDBs: 6hv9
Q-score: 0.231
Goswami P, Abid Ali F, Douglas ME, Locke J, Purkiss A, Janska A, Eickhoff P, Early A, Nans A, Cheung AMC, Diffley JFX, Costa A
Nat Commun (2018) 9 pp. 5061-5061 [ Pubmed: 30498216 DOI: doi:10.1038/s41467-018-07417-1 ]
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Nucleic acid (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Cmg-pol epsilon (Complex from Saccharomyces cerevisiae)
- Dna (5'-d(p*tp*ap*ap*ap*ap*cp*gp*gp*cp*cp*ap*gp*cp*gp*tp*gp*gp*cp*tp*gp*c)-3') (6 kDa, DNA from Saccharomyces cerevisiae)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*t)-3') (2 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Cmg-pol epsilon-dna (Complex)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna (5'-d(*gp*cp*ap*gp*cp*cp*ap*cp*gp*cp*tp*gp*gp*cp*cp*gp*tp*tp*tp*tp*a)-3') (6 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
Full-length complex of CMG helicase with polymerase epsilon
Zhou JC, Janska A, Goswami P, Renault L, Abid Ali F, Kotecha A, Diffley JFX, Costa A
PNAS (2017) 114 pp. 4141-4146 [ DOI: doi:10.1073/pnas.1700530114 Pubmed: 28373564 ]
Complex of CMG helicase with polymerase epsilon lacking the catalytic domain of Pol2
Zhou JC, Janska A, Goswami P, Renault L, Abid Ali F, Kotecha A, Diffley JFX, Costa A
PNAS (2017) 114 pp. 4141-4146 [ DOI: doi:10.1073/pnas.1700530114 Pubmed: 28373564 ]
Full-length CMG helicase complex
Zhou JC, Janska A, Goswami P, Renault L, Abid Ali F, Kotecha A, Diffley JFX, Costa A
PNAS (2017) 114 pp. 4141-4146 [ DOI: doi:10.1073/pnas.1700530114 Pubmed: 28373564 ]