Cryo-EM structure of complement C5 in complex with nanobodies UNbC5-1 and UNbC5-2
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8cml
Q-score: 0.415
Struijf EM, De la O Becerra KI, Ruyken M, de Haas CJC, van Oosterom F, Siere DY, van Keulen JE, Heesterbeek DAC, Dolk E, Heukers R, Bardoel BW, Gros P, Rooijakkers SHM
J Biol Chem (2023) 299 pp. 104956-104956 [ Pubmed: 37356719 DOI: doi:10.1016/j.jbc.2023.104956 ]
- Complement c5 alpha chain (112 kDa, Protein from Homo sapiens)
- Nanobody unbc5-2 (16 kDa, Protein from Lama glama)
- Complement c5 beta chain (73 kDa, Protein from Homo sapiens)
- Nanobody unbc5-1 (16 kDa, Protein from Lama glama)
- Complement c5 bound to nanobodies unbc5-1 and unbc5-2 (218 kDa, Complex from Homo sapiens)
Sci Adv (2022) 8 [ Pubmed: 36351012 DOI: 10.1126/sciadv.add3677 ]
Image sets:
Sci Adv (2022) 8 [ Pubmed: 36351012 DOI: 10.1126/sciadv.add3677 ]
Image sets:
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7zw1
Q-score: 0.374
Sci Adv (2022) 8 pp. eadd3677-eadd3677 [ Pubmed: 36351012 DOI: doi:10.1126/sciadv.add3677 ]
- Rod outer segment membrane protein 1 (37 kDa, Protein from Homo sapiens)
- Peripherin-2 (40 kDa, Protein from Homo sapiens)
- Nanobody (Complex from Lama)
- Human prph2-rom1 hetero-dimer (Complex from Homo sapiens)
- Human prph2-rom1 hetero-dimer (76 MDa, Complex)
- Nanobody (14 kDa, Protein from Lama)
Sci Adv (2022) 8 pp. eadd3677-eadd3677 [ Pubmed: 36351012 DOI: doi:10.1126/sciadv.add3677 ]
Sci Adv (2022) 8 pp. eadd3677-eadd3677 [ Pubmed: 36351012 DOI: doi:10.1126/sciadv.add3677 ]
Sci Adv (2022) 8 pp. eadd3677-eadd3677 [ Pubmed: 36351012 DOI: doi:10.1126/sciadv.add3677 ]
Cryo-EM structure of complement C4b in complex with nanobody E3
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 7b2m
Q-score: 0.445
De la O Becerra KI, Oosterheert W, van den Bos RM, Xenaki KT, Lorent JH, Ruyken M, Schouten A, Rooijakkers SHM, van Bergen En Henegouwen PMP, Gros P
J Immunol. (2022) 208 pp. 2207-2219 [ Pubmed: 35428691 DOI: doi:10.4049/jimmunol.2100647 ]
- Complement c4 beta chain (72 kDa, Complex from Homo sapiens)
- Anti-c4b nanobody e3 (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complement c4 alpha chain (84 kDa, Complex from Homo sapiens)
- Nanobody e3 (14 kDa, Complex from Lama glama)
- Complement c4b bound to nanobody e3 (203 kDa, Complex)
- Complement c4 beta chain (71 kDa, Protein from Homo sapiens)
- Complement c4 gamma chain (33 kDa, Protein from Homo sapiens)
- Complement c4 gamma chain (33 kDa, Complex from Homo sapiens)
- Complement c4 alpha chain (84 kDa, Protein from Homo sapiens)
Cryo-EM structure of complement C4b in complex with nanobody B5
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 7b2p
Q-score: 0.411
De la O Becerra KI, Oosterheert W, van den Bos RM, Xenaki KT, Lorent JH, Ruyken M, Schouten A, Rooijakkers SHM, van Bergen En Henegouwen PMP, Gros P
J Immunol. (2022) 208 pp. 2207-2219 [ Pubmed: 35428691 DOI: doi:10.4049/jimmunol.2100647 ]
- Complement c4 beta chain (72 kDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complement c4 alpha chain (84 kDa, Complex from Homo sapiens)
- Complement c4 beta chain (71 kDa, Protein from Homo sapiens)
- Complement c4b bound to nanobody b5 (204 kDa, Complex)
- Complement c4 gamma chain (33 kDa, Protein from Homo sapiens)
- Complement c4 gamma chain (33 kDa, Complex from Homo sapiens)
- Nanobody b5 (15 kDa, Protein from Lama glama)
- Nanobody b5 (15 kDa, Complex from Lama glama)
- Complement c4 alpha chain (84 kDa, Protein from Homo sapiens)
Cryo-EM structure of complement C4b in complex with nanobody B12
Resolution: 3.76 Å
EM Method: Single-particle
Fitted PDBs: 7b2q
Q-score: 0.371
De la O Becerra KI, Oosterheert W, van den Bos RM, Xenaki KT, Lorent JH, Ruyken M, Schouten A, Rooijakkers SHM, van Bergen En Henegouwen PMP, Gros P
J Immunol. (2022) 208 pp. 2207-2219 [ Pubmed: 35428691 DOI: doi:10.4049/jimmunol.2100647 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Anti-c4b nanobody b12 (15 kDa, Protein from Lama glama)
- Nanobody b12 (15 kDa, Complex from Lama glama)
- Complement c4b bound to nanobody b12 (204 kDa, Complex)
- Complement c4 beta chain (72 kDa, Complex from Homo sapiens)
- Complement c4 gamma chain (33 kDa, Protein from Homo sapiens)
- Complement c4 alpha chain (84 kDa, Complex from Homo sapiens)
- Complement c4 alpha chain (84 kDa, Protein from Homo sapiens)
- Complement c4 gamma chain (33 kDa, Complex from Homo sapiens)
- Complement c4 beta chain (71 kDa, Protein from Homo sapiens)