Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
Fzd4/DEP complex (local refined)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8kae
Q-score: 0.509
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Nbfab chain l (23 kDa, Protein from synthetic construct)
- 3-[3-(4-decylphenyl)-1,2,4-oxadiazol-5-yl]propan-1-amine (343 Da, Ligand)
- Anti-fab nb (13 kDa, Protein from Lama glama)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Tc-nb 8 (14 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- Nbfab-h chain (26 kDa, Protein from synthetic construct)
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7yub
Q-score: 0.513
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Nbfab-l-chain (23 kDa, Protein from synthetic construct)
- Nbfab-h-chain (26 kDa, Protein from synthetic construct)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- (2s,3r,4e)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate (379 Da, Ligand)
- Spns2 nanobody complex (Complex from Lama glama)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7yud
Q-score: 0.53
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- (2~{s})-2-azanyl-4-(4-octylphenyl)-2-[[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxymethyl]butan-1-ol (387 Da, Ligand)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)
- Nbfab-h-chain (26 kDa, Protein from synthetic construct)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7yuf
Q-score: 0.458
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Antifab nanobody (13 kDa, Protein from Lama glama)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- Nbfab h-chain (26 kDa, Protein from synthetic construct)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ydh
Q-score: 0.437
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023) 30 pp. 1343-1353.e5 [ Pubmed: 37673067 DOI: doi:10.1016/j.chembiol.2023.08.003 ]
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (130 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g protein-coupled receptor e5 subunit beta (34 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 7ydm
Q-score: 0.54
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023) 30 pp. 1343-1353.e5 [ Pubmed: 37673067 DOI: doi:10.1016/j.chembiol.2023.08.003 ]
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Gpcr/g-protein complex (130 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g protein-coupled receptor e5 subunit beta (34 kDa, Protein from Homo sapiens)
- Engineered mini-gaq (41 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
Cryo-EM structure of CD97/miniGs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ydp
Q-score: 0.357
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023) 30 pp. 1343-1353.e5 [ Pubmed: 37673067 DOI: doi:10.1016/j.chembiol.2023.08.003 ]
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Gpcr/g-protein complex (130 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g protein-coupled receptor e5 subunit beta (34 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Engineered minigas (41 kDa, Protein from Homo sapiens)
DHA-bound FFAR4 in complex with Gs
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8h4i
Q-score: 0.574
Yin H, Inoue A, Ma Z, Zhu X, Xia R, Xu Z, Wang N, Duan Y, Zhang A, Guo C, He Y
Cell Res (2023) 33 pp. 644-647 [ DOI: doi:10.1038/s41422-023-00835-x Pubmed: 37286793 ]
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Engineered mini galpha-s subunit (41 kDa, Protein from Homo sapiens)
- Docosa-4,7,10,13,16,19-hexaenoic acid (328 Da, Ligand)
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)