Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
Fzd4/DEP complex (local refined)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
Structure of human TRPV4 with antagonist A1
Resolution: 3.74 Å
EM Method: Single-particle
Fitted PDBs: 8ju5
Q-score: 0.374
Fan J, Guo C, Liao D, Ke H, Lei J, Xie W, Tang Y, Tominaga M, Huang Z, Lei X
Adv Sci (2024) 11 pp. e2401583-e2401583 [ DOI: doi:10.1002/advs.202401583 Pubmed: 38659239 ]
- Transient receptor potential cation channel subfamily v member 4,3c-gfp (128 kDa, Protein from synthetic construct)
- Trpv4 (400 kDa, Complex from Homo sapiens)
- 4-[(3~{s},4~{s})-4-(aminomethyl)-1-(5-chloranylpyridin-2-yl)sulfonyl-4-oxidanyl-pyrrolidin-3-yl]oxy-2-fluoranyl-benzenecarbonitrile (426 Da, Ligand)
Structure of human TRPV4 with antagonist GSK279
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 8ju6
Q-score: 0.415
Fan J, Guo C, Liao D, Ke H, Lei J, Xie W, Tang Y, Tominaga M, Huang Z, Lei X
Adv Sci (2024) 11 pp. e2401583-e2401583 [ DOI: doi:10.1002/advs.202401583 Pubmed: 38659239 ]
- 1-({(5s,7s)-3-[5-(2-hydroxypropan-2-yl)pyrazin-2-yl]-7-methyl-2-oxo-1-oxa-3-azaspiro[4.5]decan-7-yl}methyl)-1h-benzimidazole-6-carbonitrile (460 Da, Ligand)
- Transient receptor potential cation channel subfamily v member 4,3c-gfp (128 kDa, Protein from synthetic construct)
- Trpv4 (400 kDa, Complex from Homo sapiens)
Structure of human TRPV4 with antagonist A2
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jvi
Q-score: 0.418
Fan J, Guo C, Liao D, Ke H, Lei J, Xie W, Tang Y, Tominaga M, Huang Z, Lei X
Adv Sci (2024) 11 pp. e2401583-e2401583 [ Pubmed: 38659239 DOI: doi:10.1002/advs.202401583 ]
- Transient receptor potential cation channel subfamily v member 4,3c-gfp (128 kDa, Protein from synthetic construct)
- Trpv4 (400 kDa, Complex from Homo sapiens)
- [6-[[4-(2,4-dimethyl-1,3-thiazol-5-yl)-1,3-thiazol-2-yl]amino]pyridin-3-yl]-[(1~{s},5~{r})-3-[5-(trifluoromethyl)pyrimidin-2-yl]-3,8-diazabicyclo[3.2.1]octan-8-yl]methanone (572 Da, Ligand)
Structure of human TRPV4 with antagonist A2 and RhoA
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8jvj
Q-score: 0.378
Fan J, Guo C, Liao D, Ke H, Lei J, Xie W, Tang Y, Tominaga M, Huang Z, Lei X
Adv Sci (2024) 11 pp. e2401583-e2401583 [ DOI: doi:10.1002/advs.202401583 Pubmed: 38659239 ]
- Transient receptor potential cation channel subfamily v member 4,3c-gfp (128 kDa, Protein from synthetic construct)
- Complex of trpv4 and rhoa (400 kDa, Complex from Homo sapiens)
- Transforming protein rhoa (21 kDa, Protein from Homo sapiens)
- [6-[[4-(2,4-dimethyl-1,3-thiazol-5-yl)-1,3-thiazol-2-yl]amino]pyridin-3-yl]-[(1~{s},5~{r})-3-[5-(trifluoromethyl)pyrimidin-2-yl]-3,8-diazabicyclo[3.2.1]octan-8-yl]methanone (572 Da, Ligand)
Cryo-EM structure of the ISFba1 TnpB-reRNA-dsDNA complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8iaz
Q-score: 0.548
Ren K, Zhou F, Zhang F, Yin M, Zhu Y, Wang S, Chen Y, Huang T, Wu Z, He J, Zhang A, Guo C, Huang Z
Cell Res (2024) 34 pp. 370-385 [ DOI: doi:10.1038/s41422-024-00952-1 Pubmed: 38575718 ]
- Rna (207-mer) (66 kDa, RNA from Firmicutes bacterium AM43-11BH)
- Transposase (44 kDa, Protein from Firmicutes bacterium AM43-11BH)
- Dna (5'-d(p*cp*cp*ap*tp*tp*ap*gp*gp*ap*gp*cp*tp*gp*ap*tp*g)-3') (4 kDa, DNA from Firmicutes bacterium AM43-11BH)
- The complex of protein with grna and target dna (Complex from Firmicutes bacterium AM43-11BH)
- Dna (5'-d(p*ap*cp*ap*tp*gp*gp*ap*cp*cp*ap*tp*cp*ap*gp*cp*tp*cp*cp*tp*ap*ap*tp*gp*g)-3') (7 kDa, DNA from Firmicutes bacterium AM43-11BH)
- Zinc ion (65 Da, Ligand)
Structure of the SPARTA complex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8j7s
Q-score: 0.416
Guo M, Zhu Y, Lin Z, Yang D, Zhang A, Guo C, Huang Z
Cell Res (2023) 33 pp. 731-734 [ DOI: doi:10.1038/s41422-023-00850-y Pubmed: 37491603 ]
- Dna (5'-d(p*tp*ap*ap*tp*ap*gp*ap*tp*tp*ap*gp*ap*gp*cp*cp*gp*tp*cp*ap*ap*tp*ap*gp*a)-3') (7 kDa, DNA from Maribacter polysiphoniae)
- Tir domain-containing protein (49 kDa, Protein from Maribacter polysiphoniae)
- Piwi domain-containing protein (57 kDa, Protein from Maribacter polysiphoniae)
- Sparta complex (Complex from Maribacter polysiphoniae)
- Rna (5'-r(p*up*gp*ap*cp*gp*gp*cp*up*cp*up*ap*ap*up*cp*up*ap*up*up*a)-3') (6 kDa, RNA from Maribacter polysiphoniae)
Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7xv3
Q-score: 0.579
Liang J, Inoue A, Ikuta T, Xia R, Wang N, Kawakami K, Xu Z, Qian Y, Zhu X, Zhang A, Guo C, Huang Z, He Y
Nat Commun (2023) 14 pp. 1012-1012 [ DOI: doi:10.1038/s41467-023-36575-0 Pubmed: 36823105 ]
- Gpr174/gs complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (2~{s})-2-$l^{4}-azanyl-3-[[(2~{r})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid (497 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Probable g-protein coupled receptor 174 (38 kDa, Protein from Homo sapiens)
- Engineered g protein subunit s (mini-gs) (41 kDa, Protein from Homo sapiens)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7x10
Q-score: 0.465
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Engineered g alpha 12 subunit (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)