Cryo-EM structure of alpha-MSH-bound melanocortin-1 receptor in complex with Gs protein and Nb35
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f4d
Q-score: 0.478
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Cryo-em structure of alpha-msh-bound melanocortin-1 receptor in complex with gs protein and nb35 (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormone (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex)
- Alpha-melanocyte-stimulating hormone (Complex)
- Nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f4f
Q-score: 0.509
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein and scfv16 (Complex from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7f4h
Q-score: 0.52
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein, nb35 and scfv16 (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Scfv16 (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Melanocortin-1 receptor (Complex from synthetic construct)
Cryo-EM structure of SHU9119-bound melanocortin-1 receptor in complex with Gs protein and Nb35
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f4i
Q-score: 0.474
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Shu9119 (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Shu9119 (Complex)
- Cryo-em structure of shu9119-bound melanocortin-1 receptor in complex with gs protein and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
ghrelin-bound ghrelin receptor in complex with Gq
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f9y
Q-score: 0.469
Wang Y, Guo S, Zhuang Y, Yun Y, Xu P, He X, Guo J, Yin W, Xu HE, Xie X, Jiang Y
Nat Commun (2021) 12 pp. 5064-5064 [ Pubmed: 34417468 DOI: doi:10.1038/s41467-021-25364-2 ]
- Soluble cytochrome b562 (74 kDa, Protein from Homo sapiens)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Engineered g-alpha-q subunit (41 kDa, Protein from Homo sapiens)
- Ghrelin-28 (3 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Ghrelin-bound ghrelin receptor in complex with gq (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
GHRP-6-bound ghrelin receptor in complex with Gq
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7f9z
Q-score: 0.363
Wang Y, Guo S, Zhuang Y, Yun Y, Xu P, He X, Guo J, Yin W, Xu HE, Xie X, Jiang Y
Nat Commun (2021) 12 pp. 5064-5064 [ Pubmed: 34417468 DOI: doi:10.1038/s41467-021-25364-2 ]
- Human ghrelin receptor (74 kDa, Protein from Homo sapiens)
- Ghrp-6-bound ghrelin receptor in complex with gq (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- 3-pyridin-4-yl-2,4-dihydro-indeno[1,2-.c.]pyrazole (873 Da, Protein from synthetic construct)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Nb35 (15 kDa, Protein from Lama glama)
- Engineered g-alpha-q (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)