Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and dsDNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yzo
Q-score: 0.491
Gut F, Kashammer L, Lammens K, Bartho JD, Boggusch AM, van de Logt E, Kessler B, Hopfner KP
Mol Cell (2022) 82 pp. 3513-3522.e6 [ Pubmed: 35987200 DOI: doi:10.1016/j.molcel.2022.07.019 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nuclease sbccd subunit d (45 kDa, Protein from Escherichia coli)
- Dna (31-mer) (37 kDa, DNA from synthetic construct)
- Dna (31-mer) (36 kDa, DNA from synthetic construct)
- Nuclease sbccd subunit c (118 kDa, Protein from Escherichia coli)
- Complex of mre11 dimer, rad50 dimer and double-stranded dna (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
Gut F, Kashammer L, Lammens K, Bartho JD, Boggusch AM, van de Logt E, Kessler B, Hopfner KP
Mol Cell (2022) 82 pp. 3513-3522.e6 [ Pubmed: 35987200 DOI: doi:10.1016/j.molcel.2022.07.019 ]
Hairpin-bound state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and a DNA hairpin
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7yzp
Q-score: 0.394
Gut F, Kashammer L, Lammens K, Bartho JD, Boggusch AM, van de Logt E, Kessler B, Hopfner KP
Mol Cell (2022) 82 pp. 3513-3522.e6 [ Pubmed: 35987200 DOI: doi:10.1016/j.molcel.2022.07.019 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna hairpin (59-mer) (23 kDa, DNA from synthetic construct)
- Complex of mre11 dimer, rad50 dimer and a dna hairpin (Complex)
- Magnesium ion (24 Da, Ligand)
- Nuclease sbccd subunit c (118 kDa, Protein from Escherichia coli)
- Nuclease sbccd subunit d (45 kDa, Protein from Escherichia coli)
- Dna hairpin (Complex from DNA molecule)
- Manganese (ii) ion (54 Da, Ligand)
- Nuclease sbccd (Complex from Escherichia coli)
C. thermophilum Ku70/80 heterodimer bound to DNA
Gut F, Kashammer L, Lammens K, Bartho JD, Boggusch AM, van de Logt E, Kessler B, Hopfner KP
Mol Cell (2022) 82 pp. 3513-3522.e6 [ Pubmed: 35987200 DOI: doi:10.1016/j.molcel.2022.07.019 ]
Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and extended dsDNA
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7z03
Q-score: 0.253
Gut F, Kashammer L, Lammens K, Bartho JD, Boggusch AM, van de Logt E, Kessler B, Hopfner KP
Mol Cell (2022) 82 pp. 3513-3522.e6 [ Pubmed: 35987200 DOI: doi:10.1016/j.molcel.2022.07.019 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (39-mer) (36 kDa, DNA from synthetic construct)
- Nuclease sbccd subunit d (45 kDa, Protein from Escherichia coli)
- Dna (39-mer) (37 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Nuclease sbccd subunit c (118 kDa, Protein from Escherichia coli)
- Complex of mre11 dimer, rad50 dimer and double-stranded dna (Complex from Escherichia coli)
- Manganese (ii) ion (54 Da, Ligand)
Resting state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ATPgS
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6s6v
Q-score: 0.486
Kashammer L, Saathoff JH, Lammens K, Gut F, Bartho J, Alt A, Kessler B, Hopfner KP
Mol Cell (2019) 76 pp. 382- [ DOI: doi:10.1016/j.molcel.2019.07.035 Pubmed: 31492634 ]
- Heterotetrameric complex of full-length mre11-rad50 in complex with atpgs (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nuclease sbccd subunit c (118 kDa, Protein from Escherichia coli)
- Manganese (ii) ion (54 Da, Ligand)
- Nuclease sbccd subunit d (45 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
Kashammer L, Saathoff JH, Lammens K, Gut F, Bartho J, Alt A, Kessler B, Hopfner KP
Mol Cell (2019) 76 pp. 382-394.e6 [ DOI: doi:10.1016/j.molcel.2019.07.035 Pubmed: 31492634 ]
The E.coli Mre11-Rad50 complex bound to ADP and dsDNA with 200A visible coiled-coils
Kashammer L, Saathoff JH, Lammens K, Gut F, Bartho J, Alt A, Kessler B, Hopfner KP
Mol Cell (2019) 76 pp. 382-394.e6 [ DOI: doi:10.1016/j.molcel.2019.07.035 Pubmed: 31492634 ]
Cutting state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and dsDNA.
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6s85
Q-score: 0.395
Kashammer L, Saathoff JH, Lammens K, Gut F, Bartho J, Alt A, Kessler B, Hopfner KP
Mol Cell (2019) 76 pp. 382- [ DOI: doi:10.1016/j.molcel.2019.07.035 Pubmed: 31492634 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dsdna (Complex from synthetic construct)
- Heterotetrameric complex of full-length mre11-rad50 (sbccd) in complex with adp and dsdna (Complex)
- Mre11-rad50 (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nuclease sbccd subunit c (118 kDa, Protein from Escherichia coli)
- Nuclease sbccd subunit d (45 kDa, Protein from Escherichia coli)
- Manganese (ii) ion (54 Da, Ligand)
- Dna (32-mer) (18 kDa, DNA from synthetic construct)
- Dna (31-mer) (18 kDa, DNA from synthetic construct)