Cryo-EM structure of human proteasome assembly intermediate half-proteasome
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8yix
Q-score: 0.589
Han Y, Han Q, Tang Q, Zhang Y, Liu K
Sci Adv (2025) 11 pp. eadr7943-eadr7943 [ DOI: doi:10.1126/sciadv.adr7943 Pubmed: 39792683 ]
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-6 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-7 (30 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-1 (26 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-5 (28 kDa, Protein from Homo sapiens)
- Proteasome maturation protein (15 kDa, Protein from Homo sapiens)
- Cryo-em structure of human proteasome assembly intermediate half-proteasome (Complex from Homo sapiens)
- Proteasome subunit beta type-3 (22 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-4 (27 kDa, Protein from Homo sapiens)
- Proteasome assembly chaperone 2 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Proteasome assembly chaperone 1 (32 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-2 (22 kDa, Protein from Homo sapiens)
Cryo-EM structure of human proteasome assembly intermediate preholo-1
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8yiy
Q-score: 0.431
Han Y, Han Q, Tang Q, Zhang Y, Liu K
Sci Adv (2025) 11 pp. eadr7943-eadr7943 [ DOI: doi:10.1126/sciadv.adr7943 Pubmed: 39792683 ]
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-6 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-7 (30 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-1 (26 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-5 (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of human proteasome assembly intermediate preholo-1 (Complex from Homo sapiens)
- Proteasome subunit beta type-4 (27 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-3 (22 kDa, Protein from Homo sapiens)
- Proteasome assembly chaperone 2 (29 kDa, Protein from Homo sapiens)
- Proteasome maturation protein (15 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Proteasome assembly chaperone 1 (32 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-2 (22 kDa, Protein from Homo sapiens)
Cryo-EM structure of human proteasome assembly intermediate preholo-2
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 8yiz
Q-score: 0.393
Han Y, Han Q, Tang Q, Zhang Y, Liu K
Sci Adv (2025) 11 pp. eadr7943-eadr7943 [ DOI: doi:10.1126/sciadv.adr7943 Pubmed: 39792683 ]
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-6 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-7 (30 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-1 (26 kDa, Protein from Homo sapiens)
- Proteasome assembly chaperone 1 (32 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-5 (28 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-4 (27 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-3 (22 kDa, Protein from Homo sapiens)
- Proteasome assembly chaperone 2 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Cryo-em structure of human proteasome assembly intermediate preholo-2 (Complex from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-2 (22 kDa, Protein from Homo sapiens)
Local refined map of VPS35L(partial)-VPS26C-SNX17
Singla A, Boesch DJ, Fung HYJ, Ngoka C, Enriquez AS, Song R, Kramer DA, Han Y, Banarer E, Lemoff A, Juneja P, Billadeau DD, Bai X, Chen Z, Turer EE, Burstein E, Chen B
Nat Commun (2024) 15 pp. 10193-10193 [ Pubmed: 39587083 DOI: doi:10.1038/s41467-024-54583-6 ]
Local refined map of VPS35L(partial)-VPS29
Singla A, Boesch DJ, Fung HYJ, Ngoka C, Enriquez AS, Song R, Kramer DA, Han Y, Banarer E, Lemoff A, Juneja P, Billadeau DD, Bai X, Chen Z, Turer EE, Burstein E, Chen B
Nat Commun (2024) 15 pp. 10193-10193 [ Pubmed: 39587083 DOI: doi:10.1038/s41467-024-54583-6 ]
Human Retriever VPS35L/VPS29/VPS26C complex bound to SNX17 peptide (Composite Map)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9au7
Q-score: 0.202
Singla A, Boesch DJ, Fung HYJ, Ngoka C, Enriquez AS, Song R, Kramer DA, Han Y, Banarer E, Lemoff A, Juneja P, Billadeau DD, Bai X, Chen Z, Turer EE, Burstein E, Chen B
Nat Commun (2024) 15 pp. 10193-10193 [ Pubmed: 39587083 DOI: doi:10.1038/s41467-024-54583-6 ]
- Trimeric complex of vps35/vps29/vps26 with excess snx17 peptide (Complex from Homo sapiens)
- Sorting nexin-17 (2 kDa, Protein from Homo sapiens)
- Vacuolar protein sorting-associated protein 29 (23 kDa, Protein from Homo sapiens)
- Vps35 endosomal protein-sorting factor-like (109 kDa, Protein from Homo sapiens)
- Vacuolar protein sorting-associated protein 26c (33 kDa, Protein from Homo sapiens)
Human Retriever VPS35L/VPS29/VPS26C complex bound to SNX17 peptide (Consensus map)
Singla A, Boesch DJ, Fung HYJ, Ngoka C, Enriquez AS, Song R, Kramer DA, Han Y, Banarer E, Lemoff A, Juneja P, Billadeau DD, Bai X, Chen Z, Turer EE, Burstein E, Chen B
Nat Commun (2024) 15 pp. 10193-10193 [ Pubmed: 39587083 DOI: doi:10.1038/s41467-024-54583-6 ]
- Trimeric complex of vps35/vps29/vps26 with excess snx17 peptide (Complex from Homo sapiens)
- Vps35 endosomal protein-sorting factor-like (Protein from Homo sapiens)
- Sorting nexin-17 (Protein from Homo sapiens)
- Vacuolar protein sorting-associated protein 29 (Protein from Homo sapiens)
- Vacuolar protein sorting-associated protein 26c (Protein from Homo sapiens)
Cryo-EM structure of mouse TRPML1 channel Y404W at 2.86 Angstrom resolution
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 9cbz
Q-score: 0.371
bioRxiv (2024) [ DOI: doi:10.1101/2024.07.04.602033 Pubmed: 39005349 ]
- Mucolipin-1 (65 kDa, Protein from Mus musculus)
- Mouse trpml1 (Complex from Mus musculus)
Cryo-EM structure of mouse PI(4,5)P2-bound TRPML1 channel at 2.46 Angstrom resolution
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 9cc2
Q-score: 0.616
bioRxiv (2024) [ DOI: doi:10.1101/2024.07.04.602033 Pubmed: 39005349 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Mouse trpml1 (Complex from Mus musculus)
- Mucolipin-1 (65 kDa, Protein from Mus musculus)
- Sphingomyelin (814 Da, Ligand)