Structure of the AcMNPV baculovirus VP39 nucleocapsid (C14 helical reconstruction)
Johnstone BA, Hardy JM, Ha J, Butkovic A, Koszalka P, Accurso C, Venugopal H, de Marco A, Krupovic M, Coulibaly F
Sci Adv (2024) 10 pp. eado2631-eado2631 [ DOI: doi:10.1126/sciadv.ado2631 Pubmed: 39693434 ]
- Major nucleocapsid protein vp39 of autographa californica multiple nucleopolyhedrovirus (Protein from Autographa californica multiple nucleopolyhedrovirus)
- Autographa californica multiple nucleopolyhedrovirus (126 MDa, Virus from Autographa californica multiple nucleopolyhedrovirus)
- C14 reconstruction of the helical portion of the nucleocapsid formed by dimers of vp39 (Complex from Autographa californica multiple nucleopolyhedrovirus)
Structure of the baculovirus major nucleocapsid protein VP39 (localised reconstruction)
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8vwh
Q-score: 0.491
Johnstone BA, Hardy JM, Ha J, Butkovic A, Koszalka P, Accurso C, Venugopal H, de Marco A, Krupovic M, Coulibaly F
To Be Published
Johnstone BA, Hardy JM, Ha J, Butkovic A, Koszalka P, Accurso C, Venugopal H, de Marco M, Krupovic M, Coulibaly F
To Be Published
The base complex of the AcMNPV baculovirus nucleocapsid (Class 1, localised reconstruction)
Resolution: 4.71 Å
EM Method: Single-particle
Fitted PDBs: 8vwi
Q-score: 0.252
Johnstone BA, Hardy JM, Ha J, Butkovic A, Koszalka P, Accurso C, Venugopal H, de Marco A, Krupovic M, Coulibaly F
To Be Published
- Zinc ion (65 Da, Ligand)
- Major capsid protein (38 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Occlusion-derived virus envelope protein e27 (33 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Capsid-associated protein vp80 (79 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Protein ac142 (55 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- 38k (ac98) protein in p143-lef5 intergenic region (38 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Protein c42 (41 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Protein ac109 (44 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Autographa californica multiple nucleopolyhedrovirus (Virus from Autographa californica multiple nucleopolyhedrovirus)
The base complex of the AcMNPV baculovirus nucleocapsid (Class 2, localised reconstruction)
Resolution: 4.78 Å
EM Method: Single-particle
Fitted PDBs: 8vwj
Q-score: 0.243
Johnstone BA, Hardy JM, Ha J, Butkovic A, Koszalka P, Accurso C, Venugopal H, de Marco A, Krupovic M, Coulibaly F
To Be Published
- Zinc ion (65 Da, Ligand)
- Major capsid protein (38 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Occlusion-derived virus envelope protein e27 (33 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Capsid-associated protein vp80 (79 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Protein ac142 (55 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Protein c42 (41 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Protein ac109 (44 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- 38k (ac98) (38 kDa, Protein from Autographa californica multiple nucleopolyhedrovirus)
- Autographa californica multiple nucleopolyhedrovirus (Virus from Autographa californica multiple nucleopolyhedrovirus)
Johnstone BA, Hardy JM, Ha J, Butkovic A, Koszalka P, Accurso C, Venugopal H, de Marco A, Krupovic M, Coulibaly F
To Be Published
Binjari virus (BinJV) complexed with pr-specific Fab 2A7
Newton ND, Hardy JM, Modhiran N, Hugo LE, Amarilla AA, Bibby S, Venugopal H, Harrison JJ, Traves RJ, Hall RA, Hobson-Peters J, Coulibaly F, Watterson D
Sci Adv (2021) 7 [ Pubmed: 33990320 DOI: doi:10.1126/sciadv.abe4507 ]
- Binjari virus (22 MDa, Virus from Binjari virus)
- Binjari virus complexed with pr-specific fab 2a7 (22 MDa, Complex)
- 2a7 fab (50 kDa, Complex from Mus musculus)
The structure of an infectious immature flavivirus redefines viral architecture and maturation
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7l30
Q-score: 0.418
Newton ND, Hardy JM, Modhiran N, Hugo LE, Amarilla AA, Bibby S, Venugopal H, Harrison JJ, Traves RJ, Hall RA, Hobson-Peters J, Coulibaly F, Watterson D
Sci Adv (2021) 7 [ Pubmed: 33990320 DOI: doi:10.1126/sciadv.abe4507 ]
- Envelope protein e (53 kDa, Protein from Binjari virus)
- Prm protein (18 kDa, Protein from Binjari virus)
- Binjari virus (22 MDa, Virus from Binjari virus)
Chimeric flavivirus between Binjari virus and Dengue virus serotype-2
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7kv8
Q-score: 0.701
Hardy JM, Newton ND, Modhiran N, Scott CAP, Venugopal H, Vet LJ, Young PR, Hall RA, Hobson-Peters J, Coulibaly F, Watterson D
Nat Commun (2021) 12 pp. 3266-3266 [ Pubmed: 34075032 DOI: doi:10.1038/s41467-021-22773-1 ]
- Envelope protein e (54 kDa, Protein from Dengue virus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- M and e from dengue virus serotype-2 isolate et300 form a complex that assembles into anti-parallel dimers, (m-e)2, in the t=3 icosahedral particle. (Complex from Dengue virus 2)
- (2s,3r,4z)-3-hydroxy-2-[(9e)-octadec-9-enoylamino]octadec-4-en-1-yl dihydrogen phosphate (643 Da, Ligand)
- Binjari virus (11 MDa, Virus from Binjari virus)
- Matrix protein m (8 kDa, Protein from Dengue virus 2)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Water (18 Da, Ligand)