Single particle Data of the activated B. subtilis ClpC arranged as a tetramer of hexamers
Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T
Cell (2022) 185 pp. 2203-2205 [ Pubmed: 35662409 DOI: 10.1016/j.cell.2022.05.009 ]
Image sets:
SSUL-gCAL mediating Synechococcus elongatus PCC 7942 M58 homo-demixing
Zang K, Wang H, Hartl FU, Hayer-Hartl M
Nat Struct Mol Biol (2021) 28 pp. 909-922 [ DOI: doi:10.1038/s41594-021-00676-5 Pubmed: 34759380 ]
Zang K, Wang H, Hartl FU, Hayer-Hartl M
Nat Struct Mol Biol (2021) 28 pp. 909-922 [ DOI: doi:10.1038/s41594-021-00676-5 Pubmed: 34759380 ]
Zang K, Wang H, Hartl FU, Hayer-Hartl M
Nat Struct Mol Biol (2021) 28 pp. 909-922 [ DOI: doi:10.1038/s41594-021-00676-5 Pubmed: 34759380 ]
Cryo-EM structure of B. subtilis ClpC (DWB mutant) hexamer bound to a substrate polypeptide
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7abr
Q-score: 0.454
Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T
Cell (2022) 185 pp. 2338- [ Pubmed: 35662409 DOI: doi:10.1016/j.cell.2022.05.009 ]
- Negative regulator of genetic competence clpc/mecb (91 kDa, Protein from Bacillus subtilis (strain 168))
- Negative regulator of genetic competence clpc/mecb (Complex from Bacillus subtilis subsp. subtilis str. 168)
- Substrate polypeptide (Complex from unidentified)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- B. subtilis clpc (dwb mutant) hexamer bound to a substrate polypeptide (Complex from Bacillus subtilis subsp. subtilis str. 168)
- Substrate polypeptide (2 kDa, Protein from unidentified)
Cryo-EM map of the activated B. subtilis ClpC arranged as a tetramer of hexamers
Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T
Cell (2022) 185 pp. 2338-2353.e18 [ Pubmed: 35662409 DOI: doi:10.1016/j.cell.2022.05.009 ]
CryoEM structure of Rubisco Activase with its substrate Rubisco from Nostoc sp. (strain PCC7120)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 6z1f
Q-score: 0.489
Flecken M, Wang H, Popilka L, Hartl FU, Bracher A, Hayer-Hartl M
Cell (2020) 183 pp. 457-473.e20 [ Pubmed: 32979320 DOI: doi:10.1016/j.cell.2020.09.010 ]
- Ribulose bisphosphate carboxylase/oxygenase activase (32 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Aaa-core of the rca hexamer from nostoc sp. pcc7120 in complex with atp/atpgammas (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 2-carboxyarabinitol-1,5-diphosphate (356 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nosrca-deltac:rubisco complex (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Rubisco complex from nostoc sp. pcc7120 in complex with inhibitor cabp (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Ribulose bisphosphate carboxylase small chain (12 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Ribulose bisphosphate carboxylase large chain (53 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
Resolution: 8.2 Å
EM Method: Single-particle
Fitted PDBs: 6z1g
Q-score: 0.125
Flecken M, Wang H, Popilka L, Hartl FU, Bracher A, Hayer-Hartl M
Cell (2020) 183 pp. 457-473.e20 [ Pubmed: 32979320 DOI: doi:10.1016/j.cell.2020.09.010 ]
- Ribulose bisphosphate carboxylase large chain (53 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Complex between rubisco activase small-subunit-like domain with rubisco from nostoc sp. (strain pcc7120) (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Ribulose bisphosphate carboxylase/oxygenase activase (10 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- (rbcl)2(rbcs)2 unit of the (rbcl)8(rbcs)8 rubisco complex (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Ribulose bisphosphate carboxylase small chain (12 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Ssul domain of rca, part of the (rca)6 hexamer (Complex from Nostoc sp. PCC 7120 = FACHB-418)
Flecken M, Wang H, Popilka L, Hartl FU, Bracher A, Hayer-Hartl M
Cell (2020) 183 pp. 457-473.e20 [ Pubmed: 32979320 DOI: doi:10.1016/j.cell.2020.09.010 ]
- Aaa-core of the rca hexamer from nostoc sp. pcc7120 in complex with atp/atpgammas (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Nosrca-deltac:rubisco complex (Complex from Nostoc sp. PCC 7120 = FACHB-418)
- Rubisco complex from nostoc sp. pcc7120 in complex with inhibitor cabp (Complex from Nostoc sp. PCC 7120 = FACHB-418)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 6tmv
Q-score: 0.335
Bracher A, Paul SS, Wang H, Wischnewski N, Hartl FU, Hayer-Hartl M
PLoS One (2020) 15 pp. e0230090-e0230090 [ DOI: doi:10.1371/journal.pone.0230090 Pubmed: 32339190 ]