Near-atomic cryo-EM structure of PRC1 bound to the microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5kmg
Q-score: 0.41
Kellogg EH, Howes S, Ti SC, Ramirez-Aportela E, Kapoor TM, Chacon P, Nogales E
PNAS (2016) 113 pp. 9430-9439 [ Pubmed: 27493215 DOI: doi:10.1073/pnas.1609903113 ]
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (48 kDa, Protein from Sus scrofa)
- Ternary complex of prc1 fragment (prc1-sc), alpha-tubulin, and beta-tubulin. (Complex)
- Magnesium ion (24 Da, Ligand)
- Protein regulator of cytokinesis 1 (15 kDa, Protein from Homo sapiens)
Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syc
Q-score: 0.426
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ DOI: doi:10.1016/j.jmb.2017.01.001 Pubmed: 28104363 ]
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5sye
Q-score: 0.372
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ DOI: doi:10.1016/j.jmb.2017.01.001 Pubmed: 28104363 ]
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Ternary complex of taxol- and peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Taxol (853 Da, Ligand)
High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syf
Q-score: 0.39
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ DOI: doi:10.1016/j.jmb.2017.01.001 Pubmed: 28104363 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Taxol (853 Da, Ligand)
Cryo-EM reconstruction of zampanolide-bound microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syg
Q-score: 0.303
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ DOI: doi:10.1016/j.jmb.2017.01.001 Pubmed: 28104363 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ternary complex of zampanolide-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- (2z,4e)-n-[(s)-[(1s,2e,5s,8e,10z,17s)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide (497 Da, Ligand)