Neurotensin receptor allosterism revealed in complex with a biased allosteric modulator
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 8fmz
Q-score: 0.542
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023) 62 pp. 1233-1248 [ DOI: doi:10.1021/acs.biochem.3c00029 Pubmed: 36917754 ]
- Neurotensin/neuromedin n (819 Da, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Minigq (28 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Neurotensin receptor type 1 (45 kDa, Protein from Rattus norvegicus)
- Ternary complex of ntsr1 minigq heterotrimer with scfv16 (147 kDa, Complex from Rattus norvegicus)
CryoEM structure of Go-coupled NTSR1 with a biased allosteric modulator
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8fn0
Q-score: 0.478
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023) 62 pp. 1233-1248 [ DOI: doi:10.1021/acs.biochem.3c00029 Pubmed: 36917754 ]
- Minigo (25 kDa, Protein from Escherichia coli)
- Neurotensin/neuromedin n (819 Da, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Neurotensin receptor type 1 (45 kDa, Protein from Rattus norvegicus)
- Ternary complex of ntsr1 minigo heterotrimer with scfv16 (147 kDa, Complex from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of Go-coupled NTSR1
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8fn1
Q-score: 0.498
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023) 62 pp. 1233-1248 [ DOI: doi:10.1021/acs.biochem.3c00029 Pubmed: 36917754 ]
- Neurotensin/neuromedin n (819 Da, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Neurotensin receptor type 1 (45 kDa, Protein from Rattus norvegicus)
- Scfv16 (28 kDa, Protein from Escherichia coli)
- Ternary complex of ntsr1 minigo heterotrimer with scfv16 (147 kDa, Complex from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)