CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 7s8l
Q-score: 0.602
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ DOI: doi:10.1038/s41586-021-04126-6 Pubmed: 34789874 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
CryoEM structure of Gi-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 7s8m
Q-score: 0.562
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ DOI: doi:10.1038/s41586-021-04126-6 Pubmed: 34789874 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mrgprx2-gi1 cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562, mas-related g-protein coupled receptor member x2 chimera (54 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7s8n
Q-score: 0.579
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ DOI: doi:10.1038/s41586-021-04126-6 Pubmed: 34789874 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
CryoEM structure of Gi-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7s8o
Q-score: 0.591
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ DOI: doi:10.1038/s41586-021-04126-6 Pubmed: 34789874 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x2 chimera (54 kDa, Protein from Homo sapiens)
- Mrgprx2-gi1 (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX4 with small molecule agonist MS47134
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7s8p
Q-score: 0.605
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ DOI: doi:10.1038/s41586-021-04126-6 Pubmed: 34789874 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Mrgprx4-gq ms47134 (Complex from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4 chimera (53 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- N-[(1r,3r,5s,7r)-3,5-dimethyltricyclo[3.3.1.1~3,7~]decane-1-carbonyl]-d-phenylalanine (355 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)