Structure of human serotonin transporter bound to small molecule '8090 in lipid nanodisc and NaCl
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7txt
Q-score: 0.549
Singh I, Seth A, Billesbolle CB, Braz J, Rodriguiz RM, Roy K, Bekele B, Craik V, Huang XP, Boytsov D, Pogorelov VM, Lak P, O'Donnell H, Sandtner W, Irwin JJ, Roth BL, Basbaum AI, Wetsel WC, Manglik A, Shoichet BK, Rudnick G
Cell (2023) 186 pp. 2160-2175.e17 [ DOI: doi:10.1016/j.cell.2023.04.010 Pubmed: 37137306 ]
- 15b8 fab light chain (11 kDa, Protein from Mus musculus)
- Human serotonin transporter in complex with fab15b8 bound to '8090 in nacl (Complex)
- Fab15b8 heavy chain (Complex from Mus musculus)
- Fab15b8 light chain (Complex from Mus musculus)
- 1-[4-(4-fluorophenyl)-1,3-thiazol-2-yl]piperazine (263 Da, Ligand)
- 15b8 fab heavy chain (13 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (61 kDa, Protein from Homo sapiens)
- Sodium-dependent serotonin transporter (Complex from Homo sapiens)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7w6p
Q-score: 0.056
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ Pubmed: 36173843 DOI: doi:10.1126/science.abn7065 ]
- N-pyridin-4-ylisoquinolin-4-amine (221 Da, Ligand)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a biased agonist
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7w7e
Q-score: 0.466
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ Pubmed: 36173843 DOI: doi:10.1126/science.abn7065 ]
- 5-(3-bicyclo[4.2.0]octa-1,3,5-trienyl)-1,2,3,6-tetrahydropyridine (185 Da, Ligand)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)