CryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8dwc
Q-score: 0.573
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ DOI: doi:10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8dwg
Q-score: 0.596
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ DOI: doi:10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-[(cyclopropanesulfonyl)amino]-n-(2-ethoxyphenyl)benzamide (360 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX1 with ligand Compound-16
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8dwh
Q-score: 0.51
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ DOI: doi:10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- N-{2-[(1-aminoisoquinolin-6-yl)oxy]-4-methylphenyl}-2-methoxybenzene-1-sulfonamide (435 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
cryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Cell Biol (2022) [ DOI: 10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
Image sets:
cryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22 & ML382
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Cell Biol (2022) [ DOI: 10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
Image sets:
cryoEM structure of Gq-coupled MRGPRX1 with Compound-16
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Cell Biol (2022) [ DOI: 10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
Image sets:
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7w6p
Q-score: 0.056
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ DOI: doi:10.1126/science.abn7065 Pubmed: 36173843 ]
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- N-pyridin-4-ylisoquinolin-4-amine (221 Da, Ligand)
Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a biased agonist
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7w7e
Q-score: 0.466
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ DOI: doi:10.1126/science.abn7065 Pubmed: 36173843 ]
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 5-(3-bicyclo[4.2.0]octa-1,3,5-trienyl)-1,2,3,6-tetrahydropyridine (185 Da, Ligand)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7ran
Q-score: 0.49
Kaplan AL, Confair DN, Kim K, Barros-Alvarez X, Rodriguiz RM, Yang Y, Kweon OS, Che T, McCorvy JD, Kamber DN, Phelan JP, Martins LC, Pogorelov VM, DiBerto JF, Slocum ST, Huang XP, Kumar JM, Robertson MJ, Panova O, Seven AB, Wetsel AQ, Wetsel WC, Irwin JJ, Skiniotis G, Shoichet BK, Roth BL, Ellman JA
Nature (2022) 610 pp. 582-591 [ DOI: doi:10.1038/s41586-022-05258-z Pubmed: 36171289 ]
- 5-hydroxytryptamine receptor 2a (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- (3r)-3-methyl-5-(1h-pyrrolo[2,3-b]pyridin-3-yl)-1,2,3,6-tetrahydropyridin-1-ium (214 Da, Ligand)
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 5-ht2ar bound to a tetrahydropyridine agonist (r-69) in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 7s8l
Q-score: 0.602
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ DOI: doi:10.1038/s41586-021-04126-6 Pubmed: 34789874 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)