CryoEM structure of miniGq-coupled hM3Dq in complex with DCZ
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8e9w
Q-score: 0.543
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m3 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 11-(4-methylpiperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (292 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Gq-coupled hm3r complex (Complex)
CryoEM structure of miniGo-coupled hM4Di in complex with DCZ
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8e9x
Q-score: 0.547
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor 4, minigo protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Minigo (25 kDa, Protein from Homo sapiens)
- 11-(4-methylpiperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (292 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Gq-coupled hm3r complex (Complex)
CryoEM structure of miniGq-coupled hM3Dq in complex with CNO
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8e9y
Q-score: 0.482
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Scfv16 (26 kDa, Protein from Homo sapiens)
- 8-chloro-11-(4-methyl-4-oxo-4lambda~5~-piperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (342 Da, Ligand)
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Gq-coupled hm3r complex (Complex)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
CryoEM structure of miniGq-coupled hM3R in complex with Iperoxo
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8e9z
Q-score: 0.531
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Gq-coupled hm3r complex (Complex)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
CryoEM structure of miniGq-coupled hM3R in complex with iperoxo (local refinement)
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8ea0
Q-score: 0.537
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Hm3r (Complex from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
CryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8dwc
Q-score: 0.573
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ DOI: doi:10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8dwg
Q-score: 0.596
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ DOI: doi:10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-[(cyclopropanesulfonyl)amino]-n-(2-ethoxyphenyl)benzamide (360 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX1 with ligand Compound-16
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8dwh
Q-score: 0.51
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ DOI: doi:10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- N-{2-[(1-aminoisoquinolin-6-yl)oxy]-4-methylphenyl}-2-methoxybenzene-1-sulfonamide (435 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
cryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Cell Biol (2022) [ DOI: 10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
Image sets:
cryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22 & ML382
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Cell Biol (2022) [ DOI: 10.1038/s41589-022-01173-6 Pubmed: 36302898 ]
Image sets: