CryoEM structure of miniGq-coupled hM3Dq in complex with CNO
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8e9y
Q-score: 0.482
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Gq-coupled hm3r complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- 8-chloro-11-(4-methyl-4-oxo-4lambda~5~-piperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (342 Da, Ligand)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
CryoEM structure of miniGq-coupled hM3R in complex with Iperoxo
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8e9z
Q-score: 0.531
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Gq-coupled hm3r complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)
CryoEM structure of miniGq-coupled hM3R in complex with iperoxo (local refinement)
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8ea0
Q-score: 0.537
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Hm3r (Complex from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)