CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant, scFv16, and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Human drd2 in complex with heterotrimeric g protein goa (k46e), scfv16, and dopamine (120 kDa, Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Human drd2 in complex with heterotrimeric g protein goa (k46e) and dopamine (120 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Dopamine Receptor D1R-Gs-Rotigotine complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8irr
Q-score: 0.513
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Water (18 Da, Ligand)
- Dopamine receptor d1r-gs-rotigotine complex (Complex from Homo sapiens)
- D(1a) dopamine receptor (55 kDa, Protein from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanoboy 35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
Dopamine Receptor D4R-Gi-Rotigotine complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8iru
Q-score: 0.485
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Dopamine receptor d4r-gi-rotigotine complex (Complex from Homo sapiens)
- D(4) dopamine receptor (83 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
Dopamine Receptor D2R-Gi-Rotigotine complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8irs
Q-score: 0.468
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Dopamine receptor d2r-gi-rotigotine complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,d(2) dopamine receptor (67 kDa, Protein from Homo sapiens)
Dopamine Receptor D3R-Gi-Rotigotine complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8irt
Q-score: 0.564
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Soluble cytochrome b562,d(3) dopamine receptor (60 kDa, Protein from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Dopamine receptor d3r-gi-rotigotine complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)
Dopamine Receptor D5R-Gs-Rotigotine complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8irv
Q-score: 0.517
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Palmitic acid (256 Da, Ligand)
- Soluble cytochrome b562,d(1b) dopamine receptor (68 kDa, Protein from Homo sapiens)
- Dopamine receptor d5r-gs-rotigotine complex (Complex from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of human angiotensin receptor AT1R in complex Gq proteins and Sar1-AngII
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f6g
Q-score: 0.504
Zhang D, Liu Y, Zaidi SA, Xu L, Zhan Y, Chen A, Guo J, Huang XP, Roth BL, Katritch V, Cherezov V, Zhang H
EMBO J (2023) 42 pp. e112940-e112940 [ DOI: doi:10.15252/embj.2022112940 Pubmed: 37038975 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sar1-angii (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (9 kDa, Protein from Homo sapiens)
- Angiotensin receptor at1r in complex with gq proteins and sar1-angii (168 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (43 kDa, Protein from Homo sapiens)
- Angiotensin receptor at1r (81 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
CryoEM structure of miniGq-coupled hM3Dq in complex with CNO
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8e9y
Q-score: 0.482
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Gq-coupled hm3r complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- 8-chloro-11-(4-methyl-4-oxo-4lambda~5~-piperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (342 Da, Ligand)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
CryoEM structure of miniGq-coupled hM3R in complex with Iperoxo
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8e9z
Q-score: 0.531
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Gq-coupled hm3r complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Muscarinic acetylcholine receptor m3 (62 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Homo sapiens)