CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant, scFv16, and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Human drd2 in complex with heterotrimeric g protein goa (k46e), scfv16, and dopamine (120 kDa, Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Human drd2 in complex with heterotrimeric g protein goa (k46e) and dopamine (120 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Dopamine Receptor D1R-Gs-Rotigotine complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8irr
Q-score: 0.513
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Water (18 Da, Ligand)
- Dopamine receptor d1r-gs-rotigotine complex (Complex from Homo sapiens)
- D(1a) dopamine receptor (55 kDa, Protein from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanoboy 35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
Dopamine Receptor D4R-Gi-Rotigotine complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8iru
Q-score: 0.485
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Dopamine receptor d4r-gi-rotigotine complex (Complex from Homo sapiens)
- D(4) dopamine receptor (83 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
Dopamine Receptor D2R-Gi-Rotigotine complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8irs
Q-score: 0.468
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Dopamine receptor d2r-gi-rotigotine complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,d(2) dopamine receptor (67 kDa, Protein from Homo sapiens)
Dopamine Receptor D5R-Gs-Rotigotine complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8irv
Q-score: 0.517
Xu P, Huang S, Krumm BE, Zhuang Y, Mao C, Zhang Y, Wang Y, Huang XP, Liu YF, He X, Li H, Yin W, Jiang Y, Zhang Y, Roth BL, Xu HE
Cell Res (2023) 33 pp. 604-616 [ DOI: doi:10.1038/s41422-023-00808-0 Pubmed: 37221270 ]
- Palmitic acid (256 Da, Ligand)
- Soluble cytochrome b562,d(1b) dopamine receptor (68 kDa, Protein from Homo sapiens)
- Dopamine receptor d5r-gs-rotigotine complex (Complex from Homo sapiens)
- Rotigotine (315 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Dopamine Receptor D3R-Gi-Pramipexole complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cmu
Q-score: 0.455
Xu P, Huang S, Mao C, Krumm BE, Zhou XE, Tan Y, Huang XP, Liu Y, Shen DD, Jiang Y, Yu X, Jiang H, Melcher K, Roth BL, Cheng X, Zhang Y, Xu HE
Mol Cell (2021) 81 pp. 1147- [ Pubmed: 33548201 DOI: doi:10.1016/j.molcel.2021.01.003 ]
- Soluble cytochrome b562,d(3) dopamine receptor (60 kDa, Protein from Homo sapiens)
- Dopamine receptor d3r-gi-pramipexole complex (Complex)
- Chimera protein of soluble cytochrome b562 and d(3) dopamine receptor (Complex from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (6s)-n6-propyl-4,5,6,7-tetrahydro-1,3-benzothiazole-2,6-diamine (211 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein (Complex from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ljc
Q-score: 0.446
Zhuang Y, Krumm B, Zhang H, Zhou XE, Wang Y, Huang XP, Liu Y, Cheng X, Jiang Y, Jiang H, Zhang C, Yi W, Roth BL, Zhang Y, Xu HE
Cell Res (2021) 31 pp. 593-596 [ Pubmed: 33750903 DOI: doi:10.1038/s41422-021-00482-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- (1r)-6-chloro-1-phenyl-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (289 Da, Ligand)
- D(1a) dopamine receptor (55 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Ly3154207 and skf-81297-bound dopamine receptor 1 in complex with gi protein (Complex from Homo sapiens)
- 2-[2,6-bis(chloranyl)phenyl]-1-[(1s,3r)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1h-isoquinolin-2-yl]ethanone (450 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
- Nanoboy 35 (14 kDa, Protein from Lama glama)
- Engineered human gs alpha subunit (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ljd
Q-score: 0.454
Zhuang Y, Krumm B, Zhang H, Zhou XE, Wang Y, Huang XP, Liu Y, Cheng X, Jiang Y, Jiang H, Zhang C, Yi W, Roth BL, Zhang Y, Xu HE
Cell Res (2021) 31 pp. 593-596 [ Pubmed: 33750903 DOI: doi:10.1038/s41422-021-00482-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- D(1a) dopamine receptor (55 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- L-dopamine (153 Da, Ligand)
- 2-[2,6-bis(chloranyl)phenyl]-1-[(1s,3r)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1h-isoquinolin-2-yl]ethanone (450 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
- Nanobody 35 (14 kDa, Protein from Lama glama)
- Ly3154207 and dopamine-bound dopamine receptor 1 in complex with gs protein (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Engineered gs protein alpha subunit (29 kDa, Protein from Homo sapiens)
Cryo-EM structure of SKF-81297-bound dopamine receptor 1 in complex with Gs protein
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7jv5
Q-score: 0.431
Zhuang Y, Xu P, Mao C, Wang L, Krumm B, Zhou XE, Huang S, Liu H, Cheng X, Huang XP, Shen DD, Xu T, Liu YF, Wang Y, Guo J, Jiang Y, Jiang H, Melcher K, Roth BL, Zhang Y, Zhang C, Xu HE
Cell (2021) 184 pp. 931-942.e18 [ DOI: doi:10.1016/j.cell.2021.01.027 Pubmed: 33571431 ]
- Skf-81297-bound dopamine receptor 1 in complex with gi protein (Complex from Homo sapiens)
- (1r)-6-chloro-1-phenyl-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (289 Da, Ligand)
- D(1a) dopamine receptor (55 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Nanobody 35 (14 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)