Cryo-EM structure of the AsCas12f-sgRNA-target DNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8j12
Q-score: 0.479
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell (2023) 186 pp. 4920-4935.e23 [ DOI: doi:10.1016/j.cell.2023.08.031 Pubmed: 37776859 ]
- Zinc ion (65 Da, Ligand)
- Rna (247-mer) (72 kDa, RNA from Acidibacillus sulfooxidans)
- Dna (38-mer) (11 kDa, DNA from Acidibacillus sulfooxidans)
- Dna (38-mer) (11 kDa, DNA from Acidibacillus sulfooxidans)
- Ascas12f-sgrna-target dna (Complex from Mycolicibacterium mucogenicum)
- Magnesium ion (24 Da, Ligand)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Acidibacillus sulfuroxidans)
Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8j1j
Q-score: 0.511
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell (2023) 186 pp. 4920-4935.e23 [ DOI: doi:10.1016/j.cell.2023.08.031 Pubmed: 37776859 ]
- Zinc ion (65 Da, Ligand)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-yham-sgrnas3-5v7-target dna (Complex from Sulfoacidibacillus thermotolerans)
- Rna (118-mer) (38 kDa, RNA from Sulfoacidibacillus thermotolerans)
- Magnesium ion (24 Da, Ligand)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Sulfoacidibacillus thermotolerans)
Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8j3r
Q-score: 0.174
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell (2023) 186 pp. 4920-4935.e23 [ DOI: doi:10.1016/j.cell.2023.08.031 Pubmed: 37776859 ]
- Rna (Complex)
- Dna (37-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Zinc ion (65 Da, Ligand)
- Rna (118-mer) (38 kDa, RNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-hkra-sgrnas3-5v7-target dna (Complex from Sulfoacidibacillus thermotolerans)
- Dna (Complex from Sulfoacidibacillus thermotolerans)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Sulfoacidibacillus thermotolerans)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Magnesium ion (24 Da, Ligand)
- Ascas12f-hkra (Complex from Sulfoacidibacillus thermotolerans)
Cryo-EM structure of the Cas12m2-crRNA-target DNA full R-loop complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8hhl
Q-score: 0.547
Omura SN, Nakagawa R, Sudfeld C, Villegas Warren R, Wu WY, Hirano H, Laffeber C, Kusakizako T, Kise Y, Lebbink JHG, Itoh Y, van der Oost J, Nureki O
Nat Struct Mol Biol (2023) 30 pp. 1172-1182 [ DOI: doi:10.1038/s41594-023-01042-3 Pubmed: 37460897 ]
- Crrna-target dna (Complex)
- Zinc ion (65 Da, Ligand)
- Ts (36-mer) (10 kDa, DNA from Mycolicibacterium mucogenicum)
- Crrna (Complex)
- Cas12m2 (Complex)
- Cas12m2-crrna-target dna (Complex from Mycolicibacterium mucogenicum)
- Crrna (56-mer) (18 kDa, RNA from Mycolicibacterium mucogenicum)
- Nts (36-mer) (11 kDa, DNA from Mycolicibacterium mucogenicum)
- Target dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Cas12m2 (66 kDa, Protein from Mycolicibacterium mucogenicum)
Cryo-EM structure of the Cas12m2-crRNA-target DNA ternary complex intermediate state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8hhm
Q-score: 0.454
Omura SN, Nakagawa R, Sudfeld C, Villegas Warren R, Wu WY, Hirano H, Laffeber C, Kusakizako T, Kise Y, Lebbink JHG, Itoh Y, van der Oost J, Nureki O
Nat Struct Mol Biol (2023) 30 pp. 1172-1182 [ DOI: doi:10.1038/s41594-023-01042-3 Pubmed: 37460897 ]
- Crrna-target dna (Complex)
- Zinc ion (65 Da, Ligand)
- Crrna (Complex)
- Cas12m2 (Complex)
- Dna (36-mer) (10 kDa, DNA from Mycolicibacterium mucogenicum)
- Cas12m2-crrna-target dna (Complex from Mycolicibacterium mucogenicum)
- Dna (36-mer) (11 kDa, DNA from Mycolicibacterium mucogenicum)
- Target dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Rna (56-mer) (18 kDa, RNA from Mycolicibacterium mucogenicum)
- Cas12m2 (66 kDa, Protein from Mycolicibacterium mucogenicum)
Cryo-EM structure of the Cas12m2-crRNA binary complex
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8hio
Q-score: 0.331
Omura SN, Nakagawa R, Sudfeld C, Villegas Warren R, Wu WY, Hirano H, Laffeber C, Kusakizako T, Kise Y, Lebbink JHG, Itoh Y, van der Oost J, Nureki O
Nat Struct Mol Biol (2023) 30 pp. 1172-1182 [ DOI: doi:10.1038/s41594-023-01042-3 Pubmed: 37460897 ]
- Cas12m2-crrna (Complex from Mycolicibacterium mucogenicum)
- Zinc ion (65 Da, Ligand)
- Cas12m2 (66 kDa, Protein from Mycolicibacterium mucogenicum)
- Crrna (Complex)
- Cas12m2 (Complex)
- Rna (56-mer) (18 kDa, RNA from Mycolicibacterium mucogenicum)
- Magnesium ion (24 Da, Ligand)