Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qrm
Q-score: 0.617
Sarewicz M, Szwalec M, Pintscher S, Indyka P, Rawski M, Pietras R, Mielecki B, Koziej L, Jaciuk M, Glatt S, Osyczka A
Sci Adv (2023) 9 pp. eadd9688-eadd9688 [ DOI: doi:10.1126/sciadv.add9688 Pubmed: 36638176 ]
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from spinach)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from spinach)
- Heme c (618 Da, Ligand)
- Cytochrome f (35 kDa, Protein from spinach)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6 (24 kDa, Protein from spinach)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from spinach)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (3 kDa, Protein from spinach)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from spinach)
- Cytochrome b6f complex (220 kDa, Complex from Spinacia oleracea)
- Beta-carotene (536 Da, Ligand)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from spinach)
Resolution: 2.13 Å
EM Method: Single-particle
Fitted PDBs: 7zyv
Q-score: 0.644
Sarewicz M, Szwalec M, Pintscher S, Indyka P, Rawski M, Pietras R, Mielecki B, Koziej L, Jaciuk M, Glatt S, Osyczka A
Sci Adv (2023) 9 pp. eadd9688-eadd9688 [ DOI: doi:10.1126/sciadv.add9688 Pubmed: 36638176 ]
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from spinach)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from spinach)
- Heme c (618 Da, Ligand)
- Cytochrome f (35 kDa, Protein from spinach)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6 (24 kDa, Protein from spinach)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from spinach)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from spinach)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from spinach)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from spinach)
- Cytochrome b6f complex (220 kDa, Complex from Spinacia oleracea)
- Beta-carotene (536 Da, Ligand)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from spinach)
Cryo-EM structure of yeast Elongator complex
Resolution: 4.35 Å
EM Method: Single-particle
Fitted PDBs: 8asv
Q-score: 0.372
Jaciuk M, Scherf D, Kaszuba K, Gaik M, Rau A, Koscielniak A, Krutyholowa R, Rawski M, Indyka P, Graziadei A, Chramiec-Glabik A, Biela A, Dobosz D, Lin TY, Abbassi NE, Hammermeister A, Rappsilber J, Kosinski J, Schaffrath R, Glatt S
Nucleic Acids Res (2023) 51 pp. 2011-2032 [ DOI: doi:10.1093/nar/gkac1232 Pubmed: 36617428 ]
- Elongator complex protein 3 (63 kDa, Protein from Saccharomyces cerevisiae)
- Elongator complex protein 5 (35 kDa, Protein from Saccharomyces cerevisiae)
- Elongator complex protein 6 (30 kDa, Protein from Saccharomyces cerevisiae)
- Elongator complex protein 1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Elongator complex protein 2 (89 kDa, Protein from Saccharomyces cerevisiae)
- Yeast elongator complex (845 kDa, Complex from Saccharomyces cerevisiae)
- Elongator complex protein 4 (51 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast Elp123 in complex with alanine tRNA
Resolution: 3.96 Å
EM Method: Single-particle
Fitted PDBs: 8asw
Q-score: 0.403
Jaciuk M, Scherf D, Kaszuba K, Gaik M, Rau A, Koscielniak A, Krutyholowa R, Rawski M, Indyka P, Graziadei A, Chramiec-Glabik A, Biela A, Dobosz D, Lin TY, Abbassi NE, Hammermeister A, Rappsilber J, Kosinski J, Schaffrath R, Glatt S
Nucleic Acids Res (2023) 51 pp. 2011-2032 [ DOI: doi:10.1093/nar/gkac1232 Pubmed: 36617428 ]
- 5'-deoxyadenosine (251 Da, Ligand)
- Yeast elp123 in complex with alanine trna (636 kDa, Complex from Saccharomyces cerevisiae)
- Elongator complex protein 1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Elongator complex protein 2 (89 kDa, Protein from Saccharomyces cerevisiae)
- Elongator complex protein 3 (63 kDa, Protein from Saccharomyces cerevisiae)
- Alanine trna (23 kDa, RNA from Saccharomyces cerevisiae)
Cryo-EM structure of yeast Elp456 subcomplex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8at6
Q-score: 0.456
Jaciuk M, Scherf D, Kaszuba K, Gaik M, Rau A, Koscielniak A, Krutyholowa R, Rawski M, Indyka P, Graziadei A, Chramiec-Glabik A, Biela A, Dobosz D, Lin TY, Abbassi NE, Hammermeister A, Rappsilber J, Kosinski J, Schaffrath R, Glatt S
Nucleic Acids Res (2023) 51 pp. 2011-2032 [ DOI: doi:10.1093/nar/gkac1232 Pubmed: 36617428 ]
- Elongator complex protein 6 (30 kDa, Protein from Saccharomyces cerevisiae)
- Elongator complex protein 5 (35 kDa, Protein from Saccharomyces cerevisiae)
- Yeast elp456 subcomplex (233 kDa, Complex from Saccharomyces cerevisiae)
- Elongator complex protein 4 (51 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of mouse Elp123 with bound SAM
Resolution: 4.01 Å
EM Method: Single-particle
Fitted PDBs: 8avg
Q-score: 0.423
Jaciuk M, Scherf D, Kaszuba K, Gaik M, Rau A, Koscielniak A, Krutyholowa R, Rawski M, Indyka P, Graziadei A, Chramiec-Glabik A, Biela A, Dobosz D, Lin TY, Abbassi NE, Hammermeister A, Rappsilber J, Kosinski J, Schaffrath R, Glatt S
Nucleic Acids Res (2023) 51 pp. 2011-2032 [ DOI: doi:10.1093/nar/gkac1232 Pubmed: 36617428 ]
- Elongator complex protein 3 (62 kDa, Protein from Mus musculus)
- Mouse elp123 with bound sam (610 kDa, Complex from Mus musculus)
- Elongator complex protein 1 (149 kDa, Protein from Mus musculus)
- Iron/sulfur cluster (351 Da, Ligand)
- S-adenosylmethionine (398 Da, Ligand)
- Elongator complex protein 2 (93 kDa, Protein from Mus musculus)
Elongator catalytic subcomplex Elp123 lobe
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qk7
Q-score: 0.416
Dauden MI, Jaciuk M, Weis F, Lin TY, Kleindienst C, Abbassi NEH, Khatter H, Krutyholowa R, Breunig KD, Kosinski J, Muller CW, Glatt S
Sci Adv (2019) 5 pp. eaaw2326-eaaw2326 [ Pubmed: 31309145 DOI: doi:10.1126/sciadv.aaw2326 ]
- Elongator complex protein 3 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Elongator complex protein 1 (153 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5'-deoxyadenosine (251 Da, Ligand)
- Elongator complex protein 2 (89 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Elongator catalytic subcomplex elp123 (621 kDa, Complex from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)