Stabilised BA.1 SARS-CoV-2 spike with H6 nanobodies in '3 up' RBD conformation
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8oyt
Q-score: 0.161
Cornish K, Huo J, Jones L, Sharma P, Thrush JW, Abdelkarim S, Kipar A, Ramadurai S, Weckener M, Mikolajek H, Liu S, Buckle I, Bentley E, Kirby A, Han X, Laidlaw SM, Hill M, Eyssen L, Norman C, Le Bas A, Clarke J, James W, Stewart JP, Carroll M, Naismith JH, Owens RJ
Open Biol (2024) 14 pp. 230252-230252 [ DOI: doi:10.1098/rsob.230252 Pubmed: 38835241 ]
- H6 nanobody (Complex from Lama glama)
- Stabilised hexapro omicron ba.1 spike trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- H6 nanobody (14 kDa, Protein from Lama glama)
- Spike glycoprotein,fibritin (140 kDa, Protein from Tequatrovirus T4)
- Complex of stabilised hexapro omicron ba.1 spike trimer with three h6 nanobodies (Complex)
Stabilised BA.1 SARS-CoV-2 spike with H6 nanobodies in '2 up 1 down' RBD conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8oyu
Q-score: 0.145
Cornish K, Huo J, Jones L, Sharma P, Thrush JW, Abdelkarim S, Kipar A, Ramadurai S, Weckener M, Mikolajek H, Liu S, Buckle I, Bentley E, Kirby A, Han X, Laidlaw SM, Hill M, Eyssen L, Norman C, Le Bas A, Clarke J, James W, Stewart JP, Carroll M, Naismith JH, Owens RJ
Open Biol (2024) 14 pp. 230252-230252 [ DOI: doi:10.1098/rsob.230252 Pubmed: 38835241 ]
- H6 nanobody (Complex from Lama glama)
- Stabilised hexapro omicron ba.1 spike trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complex of stabilised hexapro omicron ba.1 spike trimer with two h6 nanobodies (Complex)
- Spike glycoprotein,fibritin (139 kDa, Protein from Enterobacteria phage T4)
- H6 nanobody (13 kDa, Protein from Lama glama)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7oan
Q-score: 0.45
Huo J, Mikolajek H, Le Bas A, Clark JJ, Sharma P, Kipar A, Dormon J, Norman C, Weckener M, Clare DK, Harrison PJ, Tree JA, Buttigieg KR, Salguero FJ, Watson R, Knott D, Carnell O, Ngabo D, Elmore MJ, Fotheringham S, Harding A, Moynie L, Ward PN, Dumoux M, Prince T, Hall Y, Hiscox JA, Owen A, James W, Carroll MW, Stewart JP, Naismith JH, Owens RJ
Nat Commun (2021) 12 pp. 5469-5469 [ DOI: doi:10.1038/s41467-021-25480-z Pubmed: 34552091 ]
- Immunoglobulin mu heavy chain (13 kDa, Protein from Vicugna pacos)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Nanobody complex (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (139 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)