Cryo-EM structure of the apo-GPR132-Gi
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8hqe
Q-score: 0.549
Wang JL, Dou XD, Cheng J, Gao MX, Xu GF, Ding W, Ding JH, Li Y, Wang SH, Ji ZW, Zhao XY, Huo TY, Zhang CF, Liu YM, Sha XY, Gao JR, Zhang WH, Hao Y, Zhang C, Sun JP, Jiao N, Yu X
Nat Metab (2023) 5 pp. 1726-1746 [ Pubmed: 37770763 DOI: doi:10.1038/s42255-023-00899-4 ]
- Soluble cytochrome b562,probable g-protein coupled receptor 132 (57 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cryo-em structure of the apo-gpr132-gi (160 MDa, Complex from Homo sapiens)
Activation mechanism of GPR132 by NPGLY
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8hqm
Q-score: 0.554
Wang JL, Dou XD, Cheng J, Gao MX, Xu GF, Ding W, Ding JH, Li Y, Wang SH, Ji ZW, Zhao XY, Huo TY, Zhang CF, Liu YM, Sha XY, Gao JR, Zhang WH, Hao Y, Zhang C, Sun JP, Jiao N, Yu X
Nat Metab (2023) 5 pp. 1726-1746 [ Pubmed: 37770763 DOI: doi:10.1038/s42255-023-00899-4 ]
- N-palmitoylglycine (313 Da, Ligand)
- Soluble cytochrome b562,probable g-protein coupled receptor 132 (57 kDa, Protein from Homo sapiens)
- Cryo-em structure of the npgly-gpr132-gi complex (160 MDa, Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Activation mechanism of GPR132 by 9(S)-HODE
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8hqn
Q-score: 0.543
Wang JL, Dou XD, Cheng J, Gao MX, Xu GF, Ding W, Ding JH, Li Y, Wang SH, Ji ZW, Zhao XY, Huo TY, Zhang CF, Liu YM, Sha XY, Gao JR, Zhang WH, Hao Y, Zhang C, Sun JP, Jiao N, Yu X
Nat Metab (2023) 5 pp. 1726-1746 [ Pubmed: 37770763 DOI: doi:10.1038/s42255-023-00899-4 ]
- (9s,10e,12z)-9-hydroxyoctadeca-10,12-dienoic acid (296 Da, Ligand)
- Soluble cytochrome b562,probable g-protein coupled receptor 132 (57 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cryo-em structure of the 9(s)hode-gpr132-gi complex (160 MDa, Complex from Homo sapiens)
Activation mechanism of GPR132 by compound NOX-6-7
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8hvi
Q-score: 0.548
Wang JL, Dou XD, Cheng J, Gao MX, Xu GF, Ding W, Ding JH, Li Y, Wang SH, Ji ZW, Zhao XY, Huo TY, Zhang CF, Liu YM, Sha XY, Gao JR, Zhang WH, Hao Y, Zhang C, Sun JP, Jiao N, Yu X
Nat Metab (2023) 5 pp. 1726-1746 [ Pubmed: 37770763 DOI: doi:10.1038/s42255-023-00899-4 ]
- Soluble cytochrome b562,probable g-protein coupled receptor 132 (57 kDa, Protein from Homo sapiens)
- 3-methyl-5-[(4-oxidanylidene-4-phenyl-butanoyl)amino]-1-benzofuran-2-carboxylic acid (351 Da, Ligand)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cryo-em structure of the apo-gpr132-gi (160 MDa, Complex from Homo sapiens)