Benchmark SBF SEM data of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy
Krentzel D, Elphick M, Domart M, Peddie CJ, Laine RF, Henriques R, Collinson LM, Jones ML
bioRxiv (2023) [ DOI: 10.1101/2023.05.11.540445 ]
Image sets:
- High magnification aligned downscaled to 14nm XY SBF SEM image stack
- High magnification aligned downscaled to 28nm XY SBF SEM image stack
- Low magnification raw SBF SEM overview images (ROI00) of the region around the cell imaged in ROI01
- High magnification aligned full resolution SBF SEM image stack
- Low magnification raw SBF SEM overview images (ROI00) of HeLa cell previously imaged by Airyscan
S. cerevisiae pol alpha - replisome complex
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8b9c
Q-score: 0.217
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911- [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
- Dna primase small subunit (51 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of replication checkpoint protein 1 (126 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna polymerase alpha catalytic subunit a (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Topoisomerase 1-associated factor 1 (141 kDa, Protein from Saccharomyces cerevisiae)
- S. cerevisiae replisome - pol alpha primase complex (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Lagging strand (32 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Leading strand (26 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome segregation in meiosis protein 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna replication complex gins protein psf3 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) [ DOI: doi:10.1016/j.molcel.2023.06.035 ]
S. cerevisiae replisome + Ctf4 + pol alpha. Unbinned, locally filtered.
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
Composite map - human replisome + Pol alpha
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
Human replisome + pol alpha. Not engaged on DNA.
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ Pubmed: 37506699 DOI: doi:10.1016/j.molcel.2023.06.035 ]
Local refinement, Pri1:Pri2-CTD. Pol alpha primase associated budding yeast replisome.
Jones ML, Aria V, Baris Y, Yeeles JTP
Mol Cell (2023) 83 pp. 2911-2924.e16 [ DOI: doi:10.1016/j.molcel.2023.06.035 Pubmed: 37506699 ]