16-nm repeating structure of A-tubule from mouse sperm flagella
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ Pubmed: 37865089 DOI: doi:10.1016/j.cell.2023.09.017 ]
16-nm repeating structure of B-tubule from mouse sperm flagella
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ Pubmed: 37865089 DOI: doi:10.1016/j.cell.2023.09.017 ]
CryoET reconstruction of 48-nm repeat doublet microtubule from human sperm
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
48 nm-repeating structure of doublets from Tektin5-KO mouse sperm axoneme
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ Pubmed: 37865089 DOI: doi:10.1016/j.cell.2023.09.017 ]
48-nm repeating structure of doublets from mouse sperm flagella
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ Pubmed: 37865089 DOI: doi:10.1016/j.cell.2023.09.017 ]
- Ef-hand calcium-binding domain-containing protein 6 (176 kDa, Protein from Mus musculus)
- Protein flattop (20 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 276 (18 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member c2 (87 kDa, Protein from Mus musculus)
- Enkurin (29 kDa, Protein from Mus musculus)
- Tektin-3 (56 kDa, Protein from Mus musculus)
- Sperm acrosome-associated protein 9 (19 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 161 (34 kDa, Protein from Mus musculus)
- Detyrosinated tubulin alpha-1a chain (50 kDa, Protein from Mus musculus)
- Coiled-coil domain-containing protein 105 (57 kDa, Protein from Mus musculus)
- Dual specificity protein phosphatase 3 (20 kDa, Protein from Mus musculus)
- Tektin-5 (62 kDa, Protein from Mus musculus)
- Tektin bundle-interacting protein 1 (23 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 95 (26 kDa, Protein from Mus musculus)
- Parkin coregulated gene protein homolog (27 kDa, Protein from Mus musculus)
- Tektin-1 (48 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member b (95 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 53 (62 kDa, Protein from Mus musculus)
- Piercer of microtubule wall 2 protein (13 kDa, Protein from Mus musculus)
- Sperm-associated antigen 8 (50 kDa, Protein from Mus musculus)
- Ef-hand domain-containing protein 1 (75 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 141 (12 kDa, Protein from Mus musculus)
- Protein fam166b (30 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 52 (68 kDa, Protein from Mus musculus)
- Tubulin beta-4b chain (49 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 107 (23 kDa, Protein from Mus musculus)
- Rib43a-like with coiled-coils protein 2 (45 kDa, Protein from Mus musculus)
- Meiosis-specific nuclear structural protein 1 (60 kDa, Protein from Mus musculus)
- Cilia- and flagella- associated protein 210 (65 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 45 (65 kDa, Protein from Mus musculus)
- Nucleoside diphosphate kinase 7 (44 kDa, Protein from Mus musculus)
- Tektin-2 (50 kDa, Protein from Mus musculus)
- Tektin-4 (52 kDa, Protein from Mus musculus)
- Piercer of microtubule wall 1 protein (18 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Mus musculus)
- Mouse sperm (Cell from Mus musculus)
Focused reconstruction of mouse doublet (register #1)
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ Pubmed: 37865089 DOI: doi:10.1016/j.cell.2023.09.017 ]
- Mouse sperm (Cell from Mus musculus)
Focused reconstruction of doublets from mouse sperm (register #2)
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ Pubmed: 37865089 DOI: doi:10.1016/j.cell.2023.09.017 ]
- Mouse sperm (Cell from Mus musculus)
Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: 10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
Image sets:
KS-AT di-domain of mycobacterial Pks13 with endogenous KS ligand bound
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 7uk4
Q-score: 0.628
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
- Acyl transferase domain (Complex from Mycolicibacterium smegmatis MC2 155)
- Polyketide synthase pks13 (198 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Ketosynthase domain (Complex from Mycolicibacterium smegmatis MC2 155)
- Unknown ligand (312 Da, Ligand)
- Water (18 Da, Ligand)
- Mycobacterial polyketide synthase 13 (Complex from Mycolicibacterium smegmatis MC2 155)
ACP1-KS-AT domains of mycobacterial Pks13
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8cuy
Q-score: 0.574
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]