Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein
Kobayashi K, Shihoya W, Nishizawa T, Kadji FMN, Aoki J, Inoue A, Nureki O
Nat. Struct. Mol. Biol. (2020) 27 pp. 274-280 [ DOI: 10.1038/s41594-020-0386-8 Pubmed: 32157248 ]
Image sets:
Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6lpb
Q-score: 0.426
Kobayashi K, Shihoya W, Nishizawa T, Kadji FMN, Aoki J, Inoue A, Nureki O
Nat Struct Mol Biol (2020) 27 pp. 274-280 [ DOI: doi:10.1038/s41594-020-0386-8 Pubmed: 32157248 ]
- Pac1r-gs trimer (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide type i receptor (46 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (4 kDa, Protein from Homo sapiens)
human Neurotensin Receptor 1 (hNTSR1) - Gi1 Protein Complex in canonical conformation (C state)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6os9
Q-score: 0.51
Kato HE, Zhang Y, Hu H, Suomivuori CM, Kadji FMN, Aoki J, Krishna Kumar K, Fonseca R, Hilger D, Huang W, Latorraca NR, Inoue A, Dror RO, Kobilka BK, Skiniotis G
Nature (2019) 572 pp. 80-85 [ Pubmed: 31243364 DOI: doi:10.1038/s41586-019-1337-6 ]
- Neurotensin receptor type 1 (48 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Hntsr1-gi1 complex in canonical conformation (c state) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Jmv449 (762 Da, Protein from synthetic construct)
- Scfv16 (27 kDa, Protein from Mus musculus)
human Neurotensin Receptor 1 (hNTSR1) - Gi1 Protein Complex in non-canonical conformation (NC state)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6osa
Q-score: 0.497
Kato HE, Zhang Y, Hu H, Suomivuori CM, Kadji FMN, Aoki J, Krishna Kumar K, Fonseca R, Hilger D, Huang W, Latorraca NR, Inoue A, Dror RO, Kobilka BK, Skiniotis G
Nature (2019) 572 pp. 80-85 [ Pubmed: 31243364 DOI: doi:10.1038/s41586-019-1337-6 ]
- Neurotensin receptor type 1 (48 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Hntsr1-gi1 complex in non-canonical conformation (nc state) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Jmv449 (762 Da, Protein from synthetic construct)