Structure of LAT1-CD98hc-Fab170 in complex with JPH203, consensus map
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 8kdd
Q-score: 0.432
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Fab170 light chain (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex)
- Fab170 (Complex from Mus musculus)
- Lat1-cd98hc (Complex from Homo sapiens)
- Nanvuranlat (472 Da, Ligand)
- Fab170 heavy chain (24 kDa, Protein from Mus musculus)
Structure of LAT1-CD98hc in complex with JPH203, focused on TMD
Resolution: 3.89 Å
EM Method: Single-particle
Fitted PDBs: 8kdf
Q-score: 0.458
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Nanvuranlat (472 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
Structure of LAT1-CD98hc-Fab170 in complex with BCH, consensus map
Resolution: 3.68 Å
EM Method: Single-particle
Fitted PDBs: 8kdg
Q-score: 0.424
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Fab170 light chain (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex)
- Fab170 (Complex from Mus musculus)
- Lat1-cd98hc (Complex from Homo sapiens)
- Fab170 heavy chain (24 kDa, Protein from Mus musculus)
- (1~{s},2~{r},4~{r})-2-azanylbicyclo[2.2.1]heptane-2-carboxylic acid (155 Da, Ligand)
Structure of LAT1-CD98hc in complex with BCH, focused on TMD
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 8kdh
Q-score: 0.45
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- (1~{s},2~{r},4~{r})-2-azanylbicyclo[2.2.1]heptane-2-carboxylic acid (155 Da, Ligand)
Structure of apo inward-open LAT1-CD98hc-Fab170 in nanodisc, consensus map
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 8kdi
Q-score: 0.43
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Fab170 light chain (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lat1-cd98hc (Complex from Homo sapiens)
- Fab170 (Complex from Mus musculus)
- Lat1-cd98hc-fab170 (Complex)
- Fab170 heavy chain (24 kDa, Protein from Mus musculus)
Structure of apo inward-open LAT1-CD98h in nanodisc, focused on TMD
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8kdj
Q-score: 0.44
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
Structure of LAT1-CD98hc in complex with L-Phe, focused on TMD
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8kdn
Q-score: 0.413
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Phenylalanine (165 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
Structure of LAT1-CD98hc in complex with melphalan, focused on TMD
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8kdo
Q-score: 0.417
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- (2~{s})-2-azanyl-3-[4-[bis(2-chloroethyl)amino]phenyl]propanoic acid (305 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
Structure of apo outward-open LAT1-CD98h in nanodisc, focused on TMD
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8kdp
Q-score: 0.409
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
Inward-facing structure of human EAAT2 in the WAY213613-bound state
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7vr7
Q-score: 0.553
Kato T, Kusakizako T, Jin C, Zhou X, Ohgaki R, Quan L, Xu M, Okuda S, Kobayashi K, Yamashita K, Nishizawa T, Kanai Y, Nureki O
Nat Commun (2022) 13 pp. 4714-4714 [ DOI: doi:10.1038/s41467-022-32442-6 Pubmed: 35953475 ]
- (2s)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid (415 Da, Ligand)
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Eaat2 (Complex from Homo sapiens)
- Excitatory amino acid transporter 2 (63 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)