Molecular mechanism of the the wake-promoting agent TAK-925
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7sr8
Q-score: 0.457
Yin J, Kang Y, McGrath AP, Chapman K, Sjodt M, Kimura E, Okabe A, Koike T, Miyanohana Y, Shimizu Y, Rallabandi R, Lian P, Bai X, Flinspach M, De Brabander JK, Rosenbaum DM
Nat Commun (2022) 13 pp. 2902-2902 [ Pubmed: 35614071 DOI: doi:10.1038/s41467-022-30601-3 ]
- A modified guanine nucleotide-binding protein g(q) subunit alpha (27 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Oleic acid (282 Da, Ligand)
- Human orexin receptor 2 - g protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Hypocretin receptor type 2 (46 kDa, Protein from Homo sapiens)
- Methyl (2r,3s)-3-[(methanesulfonyl)amino]-2-({[(1s,4s)-4-phenylcyclohexyl]oxy}methyl)piperidine-1-carboxylate (424 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (9 kDa, Protein from Homo sapiens)
Structure of the orexin-2 receptor (OX2R) bound to TAK-925, Gi and scFv16
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 7sqo
Q-score: 0.487
Yin J, Kang Y, McGrath AP, Chapman K, Sjodt M, Kimura E, Okabe A, Koike T, Miyanohana Y, Shimizu Y, Rallabandi R, Lian P, Bai X, Flinspach M, De Brabander JK, Rosenbaum DM
Nat Commun (2022) 13 pp. 2902-2902 [ Pubmed: 35614071 DOI: doi:10.1038/s41467-022-30601-3 ]
- Orexin receptor type 2 (Complex from Homo sapiens)
- Human ox2r in complex with gai/gb/gg-scfv16. (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Oleic acid (282 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Bos taurus)
- Scfv-16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Methyl (2r,3s)-3-[(methanesulfonyl)amino]-2-({[(1s,4s)-4-phenylcyclohexyl]oxy}methyl)piperidine-1-carboxylate (424 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Orexin receptor type 2 (50 kDa, Protein from Homo sapiens)
- Scfv-16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Bos taurus)
CryoEM structure of Abo1 hexamer - ADP complex
Resolution: 4.44 Å
EM Method: Single-particle
Fitted PDBs: 6jpq
Q-score: 0.188
Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ
Nat Commun (2019) 10 pp. 5764-5764 [ DOI: doi:10.1038/s41467-019-13743-9 Pubmed: 31848341 ]
CryoEM structure of human alpha4beta2 nicotinic acetylcholine receptor in complex with varenicline
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 6ur8
Q-score: 0.448
Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA
Nat Commun (2020) 11 pp. 1598-1598 [ Pubmed: 32221310 DOI: doi:10.1038/s41467-020-15363-0 ]
- Beta-d-mannopyranose (180 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Varenicline (211 Da, Ligand)
- Chimera of soluble cytochrome b562 (bril) and neuronal acetylcholine receptor subunit alpha-4 (56 kDa, Protein from Homo sapiens)
- Human alpha4beta2 nicotinic acetylcholine receptor in complex fab fragments and bound to varenicline (Complex from Homo sapiens)
- Neuronal acetylcholine receptor subunit beta-2 (45 kDa, Protein from Homo sapiens)
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 6usf
Q-score: 0.394
Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA
Nat Commun (2020) 11 pp. 1598-1598 [ Pubmed: 32221310 DOI: doi:10.1038/s41467-020-15363-0 ]
- Beta-d-mannopyranose (180 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab light chain (23 kDa, Protein from Homo sapiens)
- Fab heavy chain (24 kDa, Protein from Homo sapiens)
- Varenicline (211 Da, Ligand)
- Chimera of soluble cytochrome b562 (bril) and neuronal acetylcholine receptor subunit alpha-4 (55 kDa, Protein from Homo sapiens)
- Human alpha4beta2 nicotinic acetylcholine receptor in complex fab fragments and bound to varenicline (Complex from Homo sapiens)
- Neuronal acetylcholine receptor subunit beta-2 (45 kDa, Protein from Homo sapiens)
Abo1 structure with AAA+ and bromodomains in apo state
Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ
Nat Commun (2019) 10 pp. 5764-5764 [ DOI: doi:10.1038/s41467-019-13743-9 Pubmed: 31848341 ]
- Abo1 (Protein from Schizosaccharomyces pombe)
- Apo-abo1 (Complex from Schizosaccharomyces pombe)
CryoEM structure of Abo1 hexamer - apo complex
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 6jpu
Q-score: 0.181
Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ
Nat Commun (2019) 10 pp. 5764-5764 [ DOI: doi:10.1038/s41467-019-13743-9 Pubmed: 31848341 ]
CryoEM structure of Abo1 Walker B (E372Q) mutant hexamer - ATP complex
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 6jq0
Q-score: 0.463
Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ
Nat Commun (2019) 10 pp. 5764-5764 [ DOI: doi:10.1038/s41467-019-13743-9 Pubmed: 31848341 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Abo1 (Complex from Saccharomyces)
- Unknown substrate (1 kDa, Protein from Spodoptera frugiperda)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Uncharacterized aaa domain-containing protein c31g5.19 (136 kDa, Protein from Schizosaccharomyces pombe 972h-)
human soluble guanylate cyclase H105C mutant
Nature (2019) 574 pp. 206-210 [ DOI: doi:10.1038/s41586-019-1584-6 Pubmed: 31514202 ]
Structure of a SUR1 fusion protein
Ding D, Wang M, Wu JX, Kang Y, Chen L
Cell Rep (2019) 27 pp. 1848-1857.e4 [ Pubmed: 31067468 DOI: doi:10.1016/j.celrep.2019.04.050 ]
- Katp (Complex from Mus musculus)