Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
Structure of gamma-tubulin in the native human gamma-tubulin ring complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6v5v
Q-score: 0.444
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin gamma-1 chain (51 kDa, Protein from Homo sapiens)
- Native human gamma-tubulin ring complex (Complex from Homo sapiens)
Structures of GCP4 and GCP5 in the native human gamma-tubulin ring complex
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6v69
Q-score: 0.425
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
- Gamma-tubulin complex component 5 (118 kDa, Protein from Homo sapiens)
- Gamma-tubulin complex component 4 (76 kDa, Protein from Homo sapiens)
- Native human gamma-tubulin ring complex (Complex from Homo sapiens)
Structures of GCP2 and GCP3 in the native human gamma-tubulin ring complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6v6b
Q-score: 0.49
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
- Gamma-tubulin complex component 2 (105 kDa, Protein from Homo sapiens)
- Native human gamma-tubulin ring complex (Complex from Homo sapiens)
- Gamma-tubulin complex component 3 (103 kDa, Protein from Homo sapiens)
Structure of GCP6 in the native human gamma-tubulin ring complex
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6v6c
Q-score: 0.436
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
- Gamma-tubulin complex component 6 (200 kDa, Protein from Homo sapiens)
- Native human gamma-tubulin ring complex (Complex from Homo sapiens)
Desnity map of lumenal bridge in reconstruction of the native human gamma-tubulin ring complex
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
Density map for overlap region in reconstruction of native human gamma-tubulin ring complex
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
Structure of the native human gamma-tubulin ring complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6v6s
Q-score: 0.218
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
- Unassigned poly-alanine model ("lumenal bridge helical bundles") (22 kDa, Protein from Homo sapiens)
- Beta actin (41 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin gamma-1 chain (51 kDa, Protein from Homo sapiens)
- Gamma-tubulin complex component 5 (118 kDa, Protein from Homo sapiens)
- Unassigned poly-alanine model ("hb") (14 kDa, Protein from Homo sapiens)
- Gamma-tubulin complex component 2 (105 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unassigned poly-alanine model ("cc") (5 kDa, Protein from Homo sapiens)
- Gamma-tubulin complex component 4 (76 kDa, Protein from Homo sapiens)
- Native human gamma-tubulin ring complex (Complex from Homo sapiens)
- Gamma-tubulin complex component 3 (103 kDa, Protein from Homo sapiens)
- Unassigned poly-alanine chain ("staple") (4 kDa, Protein from Homo sapiens)
- Gamma-tubulin complex component 6 (200 kDa, Protein from Homo sapiens)
Overall density map of the native human gamma-tubulin ring complex
Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM
Cell (2020) 180 pp. 165-175.e16 [ Pubmed: 31862189 DOI: doi:10.1016/j.cell.2019.12.007 ]
13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules decorated with kinesin-1 motor domain
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 6e7b
Q-score: 0.43
Dev Cell (2018) 47 pp. 175- [ DOI: doi:10.1016/j.devcel.2018.08.014 Pubmed: 30245156 ]
- Magnesium ion (24 Da, Ligand)
- 13-pf 3-start gmpcpp-human alpha1b/beta3 microtubule decorated with kinesin-1 motor domain (Complex from Homo sapiens)
- Tubulin beta-3 chain (47 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)