cryo-EM structure of GPR6-Gs-Nb35 complex
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8tyw
Q-score: 0.398
Barekatain M, Johansson LC, Lam JH, Chang H, Sadybekov AV, Han GW, Russo J, Bliesath J, Brice NL, Carlton MBL, Saikatendu KS, Sun H, Murphy ST, Monenschein H, Schiffer HH, Popov P, Lutomski CA, Robinson CV, Liu ZJ, Hua T, Katritch V, Cherezov V
Sci Signal (2024) 17 pp. eado8741-eado8741 [ DOI: doi:10.1126/scisignal.ado8741 Pubmed: 39626010 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 6 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Gpr6-gs-nb35 complex (Complex from Homo sapiens)
Cryo-EM structure of Dopamine 3 Receptor:Go complex bound to bitopic FOB02-04A - Conformation A
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 9f33
Q-score: 0.528
Arroyo-Urea S, Nazarova AL, Carrion-Antoli A, Bonifazi A, Battiti FO, Lam JH, Newman AH, Katritch V, Garcia-Nafria J
Nat Commun (2024) 15 pp. 7759-7759 [ Pubmed: 39237617 DOI: doi:10.1038/s41467-024-51993-4 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- N-[2-[(1r,2s)-2-[[(2s,5s)-2-(6-azanylpyridin-3-yl)-5-methyl-morpholin-4-yl]methyl]cyclopropyl]ethyl]-1h-indole-2-carboxamide (433 Da, Ligand)
- Antibody scfv16 (28 kDa, Protein from Mus musculus)
- Green fluorescent protein,d(3) dopamine receptor (74 kDa, Protein from Homo sapiens)
- D3r:go protein complex with bitopic fob02-04a-conformation a (190 kDa, Complex from Homo sapiens)
Cryo-EM structure of Dopamine 3 receptor:Go complex bound to bitopic FOB02-04A - Conformation B
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 9f34
Q-score: 0.456
Arroyo-Urea S, Nazarova AL, Carrion-Antoli A, Bonifazi A, Battiti FO, Lam JH, Newman AH, Katritch V, Garcia-Nafria J
Nat Commun (2024) 15 pp. 7759-7759 [ Pubmed: 39237617 DOI: doi:10.1038/s41467-024-51993-4 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- N-[2-[(1r,2s)-2-[[(2s,5s)-2-(6-azanylpyridin-3-yl)-5-methyl-morpholin-4-yl]methyl]cyclopropyl]ethyl]-1h-indole-2-carboxamide (433 Da, Ligand)
- Antibody scfv16 (27 kDa, Protein from Mus musculus)
- Green fluorescent protein,d(3) dopamine receptor (74 kDa, Protein from Homo sapiens)
- D3r: gtrimer: scfv16 complex bound to the bitopic fob02-04a - conformation b (190 kDa, Complex from Homo sapiens)
momSalB bound Kappa Opioid Receptor in complex Gi1
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ Pubmed: 37138078 DOI: doi:10.1038/s41586-023-06030-7 ]
GR89,696 bound Kappa Opioid Receptor in complex with Gz
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ Pubmed: 37138078 DOI: doi:10.1038/s41586-023-06030-7 ]
GR89,696 bound Kappa Opioid Receptor in complex with gustducin
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ Pubmed: 37138078 DOI: doi:10.1038/s41586-023-06030-7 ]
Cryo-EM structure of GPR156 of GPR156-miniGo-scFv16 complex (local refine)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8ieb
Q-score: 0.435
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)
- Gpr156 (131 MDa, Complex from Homo sapiens)
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)
Cryo-EM structure of miniGo-scFv16 of GPR156-miniGo-scFv16 complex (local refine)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8iec
Q-score: 0.412
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
- Minigo-scfv16 (27 MDa, Complex from Homo sapiens)
- Minigo heterotrimeric complex (Complex from Homo sapiens)
Cryo-EM structure of GPR156-miniGo-scFv16 complex
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8ied
Q-score: 0.402
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ DOI: doi:10.1038/s41594-024-01224-7 Pubmed: 38332368 ]
- Scfv16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)
- Gpr156-minigo-scfv16 complex (27 MDa, Complex from Homo sapiens)
- Gpr156 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Minigo heterotrimeric complex (Complex from Homo sapiens)
Cryo-EM structure of GPR156A/B of G-protein free GPR156 (local refine)
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8iei
Q-score: 0.507
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Gpr156a/b (131 MDa, Complex from Homo sapiens)
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)