CryoEM Structure of HCA2-Gi1 in complex with MK-1903
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8utd
Q-score: 0.347
Kang HJ, Krumm BE, Tassou A, Geron M, DiBerto JF, Kapolka NJ, Gumpper RH, Sakamoto K, Dewran Kocak D, Olsen RHJ, Huang XP, Zhang S, Huang KL, Zaidi SA, Nguyen MT, Jo MJ, Katritch V, Fay JF, Scherrer G, Roth BL
Cell (2024) 187 pp. 7433-7449.e20 [ Pubmed: 39631393 DOI: doi:10.1016/j.cell.2024.11.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Human hca2-gai1 in complex with mk1903 (Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- (4ar,5ar)-4,4a,5,5a-tetrahydro-1h-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole-3-carboxylic acid (164 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Hydroxycarboxylic acid receptor 2 (42 kDa, Protein from Homo sapiens)
CryoEM Structure of HCA2 DREADD Gi1 in complex with FCH-2296413
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8uuj
Q-score: 0.53
Kang HJ, Krumm BE, Tassou A, Geron M, DiBerto JF, Kapolka NJ, Gumpper RH, Sakamoto K, Dewran Kocak D, Olsen RHJ, Huang XP, Zhang S, Huang KL, Zaidi SA, Nguyen MT, Jo MJ, Katritch V, Fay JF, Scherrer G, Roth BL
Cell (2024) 187 pp. 7433-7449.e20 [ Pubmed: 39631393 DOI: doi:10.1016/j.cell.2024.11.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Mouse hcad-gai1 in complex with fch-2296413 (Complex from Homo sapiens)
- (3m,4ar,5ar)-3-(1h-tetrazol-5-yl)-4,4a,5,5a-tetrahydro-1h-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole (188 Da, Ligand)
- Hydroxycarboxylic acid receptor 2 (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
cryo-EM structure of GPR6-Gs-Nb35 complex
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8tyw
Q-score: 0.398
Barekatain M, Johansson LC, Lam JH, Chang H, Sadybekov AV, Han GW, Russo J, Bliesath J, Brice NL, Carlton MBL, Saikatendu KS, Sun H, Murphy ST, Monenschein H, Schiffer HH, Popov P, Lutomski CA, Robinson CV, Liu ZJ, Hua T, Katritch V, Cherezov V
Sci Signal (2024) 17 pp. eado8741-eado8741 [ Pubmed: 39626010 DOI: doi:10.1126/scisignal.ado8741 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Gpr6-gs-nb35 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 6 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of Dopamine 3 Receptor:Go complex bound to bitopic FOB02-04A - Conformation A
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 9f33
Q-score: 0.528
Arroyo-Urea S, Nazarova AL, Carrion-Antoli A, Bonifazi A, Battiti FO, Lam JH, Newman AH, Katritch V, Garcia-Nafria J
Nat Commun (2024) 15 pp. 7759-7759 [ DOI: doi:10.1038/s41467-024-51993-4 Pubmed: 39237617 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Green fluorescent protein,d(3) dopamine receptor (74 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- N-[2-[(1r,2s)-2-[[(2s,5s)-2-(6-azanylpyridin-3-yl)-5-methyl-morpholin-4-yl]methyl]cyclopropyl]ethyl]-1h-indole-2-carboxamide (433 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
- D3r:go protein complex with bitopic fob02-04a-conformation a (190 kDa, Complex from Homo sapiens)
- Antibody scfv16 (28 kDa, Protein from Mus musculus)
Cryo-EM structure of Dopamine 3 receptor:Go complex bound to bitopic FOB02-04A - Conformation B
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 9f34
Q-score: 0.456
Arroyo-Urea S, Nazarova AL, Carrion-Antoli A, Bonifazi A, Battiti FO, Lam JH, Newman AH, Katritch V, Garcia-Nafria J
Nat Commun (2024) 15 pp. 7759-7759 [ DOI: doi:10.1038/s41467-024-51993-4 Pubmed: 39237617 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Green fluorescent protein,d(3) dopamine receptor (74 kDa, Protein from Homo sapiens)
- Antibody scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- N-[2-[(1r,2s)-2-[[(2s,5s)-2-(6-azanylpyridin-3-yl)-5-methyl-morpholin-4-yl]methyl]cyclopropyl]ethyl]-1h-indole-2-carboxamide (433 Da, Ligand)
- D3r: gtrimer: scfv16 complex bound to the bitopic fob02-04a - conformation b (190 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of GPR156 of GPR156-miniGo-scFv16 complex (local refine)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8ieb
Q-score: 0.435
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Gpr156 (131 MDa, Complex from Homo sapiens)
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)
Cryo-EM structure of miniGo-scFv16 of GPR156-miniGo-scFv16 complex (local refine)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8iec
Q-score: 0.412
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Minigo heterotrimeric complex (Complex from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
- Minigo-scfv16 (27 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
Cryo-EM structure of GPR156-miniGo-scFv16 complex
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8ied
Q-score: 0.402
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Gpr156 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)
- Gpr156-minigo-scfv16 complex (27 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Minigo heterotrimeric complex (Complex from Homo sapiens)
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
- Scfv16 (Complex from Mus musculus)
Cryo-EM structure of GPR156A/B of G-protein free GPR156 (local refine)
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8iei
Q-score: 0.507
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)
- Gpr156a/b (131 MDa, Complex from Homo sapiens)
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)
Cryo-EM structure of GPR156C/D of G-protein free GPR156 (local refine)
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8iep
Q-score: 0.511
Shin J, Park J, Jeong J, Lam JH, Qiu X, Wu D, Kim K, Lee JY, Robinson CV, Hyun J, Katritch V, Kim KP, Cho Y
Nat Struct Mol Biol (2024) 31 pp. 678-687 [ Pubmed: 38332368 DOI: doi:10.1038/s41594-024-01224-7 ]
- Gpr156c/d (131 MDa, Complex from Homo sapiens)
- Probable g-protein coupled receptor 156 (65 kDa, Protein from Homo sapiens)
- [(2r)-3-[(e)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate (760 Da, Ligand)