Cryo-EM Structure of the P74-26 Tail Tube
Resolution: 2.72 Å
EM Method: Helical reconstruction
Fitted PDBs: 8ed0
Q-score: 0.64
Agnello E, Pajak J, Liu X, Kelch BA
J Biol Chem (2023) 299 pp. 103021-103021 [ DOI: doi:10.1016/j.jbc.2023.103021 Pubmed: 36791911 ]
- Oshimavirus p7426 (Virus from Oshimavirus P7426)
- Tail tube protein gp93 (37 kDa, Protein from Oshimavirus P7426)
Cryo-EM Structure of P74-26 tail-like tubes
Resolution: 2.81 Å
EM Method: Helical reconstruction
Fitted PDBs: 8edx
Q-score: 0.634
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7u19
Q-score: 0.419
Liu X, Gaubitz C, Pajak J, Kelch BA
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77483 Pubmed: 35731107 ]
- Magnesium ion (24 Da, Ligand)
- Dna (3 kDa, DNA from synthetic construct)
- Rfc bound to pcna and dna with a nick (367 kDa, Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (10 kDa, DNA from synthetic construct)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (6 kDa, DNA from synthetic construct)
RFC:PCNA bound to dsDNA with a ssDNA gap of six nucleotides
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7u1a
Q-score: 0.491
Liu X, Gaubitz C, Pajak J, Kelch BA
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77483 Pubmed: 35731107 ]
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Dna - primer (7 kDa, DNA from synthetic construct)
- Rfc bound to pcna and dna with a ssdna gap of six nucleotides (365 kDa, Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dna - template (15 kDa, DNA from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna - non primer (6 kDa, DNA from synthetic construct)
RFC:PCNA bound to DNA with a ssDNA gap of five nucleotides
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7u1p
Q-score: 0.526
Liu X, Gaubitz C, Pajak J, Kelch BA
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77483 Pubmed: 35731107 ]
- Rfc bound to pcna and dna with a ssdna gap of five nucleotides (364 kDa, Complex from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dna - primer (7 kDa, DNA from synthetic construct)
- Dna - template (15 kDa, DNA from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna - non primer (6 kDa, DNA from synthetic construct)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7thj
Q-score: 0.423
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch BA
eLife (2022) 11 [ DOI: doi:10.7554/eLife.74175 Pubmed: 35179493 ]
- Rfc2 (Protein from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Rfc5 (Protein from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Replication factor c (rfc), the eukaryotic clamp loader (336 kDa, Complex from Saccharomyces cerevisiae)
- Pcna (Protein from Saccharomyces cerevisiae)
- Rfc3 (RNA from Saccharomyces cerevisiae)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6vvo
Q-score: 0.515
Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA
bioRxiv (2020) [ DOI: doi:10.1101/2020.02.18.953257 ]
- Replication factor c subunit 3 (40 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 4 (39 kDa, Protein from Homo sapiens)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human replication factor c (rfc) complex bound to the sliding clamp proliferating cell nuclear antigen (pcna) (310 kDa, Complex from Homo sapiens)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
- Replication factor c subunit 5 (38 kDa, Protein from Homo sapiens)
- Replication factor c subunit 1 (66 kDa, Protein from Homo sapiens)
- Replication factor c subunit 2 (39 kDa, Protein from Homo sapiens)