Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ DOI: doi:10.1101/2024.12.27.630464 Pubmed: 39763753 ]
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ DOI: doi:10.1101/2024.12.27.630464 Pubmed: 39763753 ]
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
Structure of Src in complex with beta-arrestin 1 revealing SH3 binding sites
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9bt8
Q-score: 0.539
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Bacteriophage sp.)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Antibody fragment fab30, light chain (23 kDa, Protein from Bacteriophage sp.)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with three-domain src (sh3-sh2-sh1). (Complex from Rattus norvegicus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (54 kDa, Protein from Gallus gallus)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9cx3
Q-score: 0.423
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9cx9
Q-score: 0.47
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
Cryo-EM structure of the human TRPC1/C4 heteromer
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8wpl
Q-score: 0.504
Won J, Kim J, Kim J, Ko J, Park CH, Jeong B, Lee SE, Jeong H, Kim SH, Park H, So I, Lee HH
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01408-1 Pubmed: 39478185 ]
- Structure of a trp channel complex, apo state (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Short transient receptor potential channel 4 (105 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Short transient receptor potential channel 1 (91 kDa, Protein from Homo sapiens)
- 2-(hexadecanoyloxy)-1-[(phosphonooxy)methyl]ethyl hexadecanoate (648 Da, Ligand)
Cryo-EM structure of the human TRPC1/C4 heteromer in complex with Pico145
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8wpm
Q-score: 0.442
Won J, Kim J, Kim J, Ko J, Park CH, Jeong B, Lee SE, Jeong H, Kim SH, Park H, So I, Lee HH
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01408-1 Pubmed: 39478185 ]
- Structure of a trp channel complex, apo state (Complex from Homo sapiens)
- Short transient receptor potential channel 4 (105 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Short transient receptor potential channel 1 (91 kDa, Protein from Homo sapiens)
- 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-[3-(trifluoromethyloxy)phenoxy]purine-2,6-dione (524 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of the human TRPC4 in lipid nanodiscs
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 8wpn
Q-score: 0.567
Won J, Kim J, Kim J, Ko J, Park CH, Jeong B, Lee SE, Jeong H, Kim SH, Park H, So I, Lee HH
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01408-1 Pubmed: 39478185 ]
- 2-(hexadecanoyloxy)-1-[(phosphonooxy)methyl]ethyl hexadecanoate (648 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Short transient receptor potential channel 4 (105 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Structure of a trp channel (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of the human P2X3 receptor-compound 26a complex
Kim GR, Kim S, Kim YO, Han X, Nagel J, Kim J, Song DI, Muller CE, Yoon MH, Jin MS, Kim YC
J Med Chem (2024) 67 pp. 14443-14465 [ DOI: doi:10.1021/acs.jmedchem.4c01214 Pubmed: 39102524 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- P2x purinoceptor 3 (40 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human p2x3 receptor-compound 26a complex (Complex from Homo sapiens)
- 4-[2-cyclopropyl-7-[[(1~{r})-1-naphthalen-2-ylethyl]amino]-[1,2,4]triazolo[1,5-a]pyrimidin-5-yl]piperazine-1-carboxamide (456 Da, Ligand)
Structure of AT118-H Nanobody Antagonist in Complex with the Angiotensin II Type I Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8th3
Q-score: 0.532
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ DOI: doi:10.1038/s41589-024-01620-6 Pubmed: 38744986 ]
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)
- At118-h nanobody, type-1 angiotensin ii receptor, soluble cytochrome b562 complex (65 kDa, Protein from Homo sapiens)
- At118-h nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion, anti-bril fab, and anti-fab nanobody (Complex from Homo sapiens)