Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
Structure of AT118-H Nanobody Antagonist in Complex with the Angiotensin II Type I Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8th3
Q-score: 0.532
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ Pubmed: 38744986 DOI: doi:10.1038/s41589-024-01620-6 ]
- At118-h nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion, anti-bril fab, and anti-fab nanobody (Complex from Homo sapiens)
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- At118-h nanobody, type-1 angiotensin ii receptor, soluble cytochrome b562 complex (65 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8th4
Q-score: 0.495
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ Pubmed: 38744986 DOI: doi:10.1038/s41589-024-01620-6 ]
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- Type-1 angiotensin ii receptor soluble cytochrome b562 complex (49 kDa, Protein from Homo sapiens)
- At118-l nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion protein, anti-bril fab, anti-fab nanobody, and losartan (Complex from Homo sapiens)
- [2-butyl-5-chloranyl-3-[[4-[2-(2h-1,2,3,4-tetrazol-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol (422 Da, Ligand)
- At118-l nanobody (15 kDa, Protein from synthetic construct)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)