cryo-EM structure of HK022 putRNA-associated E.coli RNA polymerase elongation complex
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 7xue
Q-score: 0.476
Hwang S, Olinares PDB, Lee J, Kim J, Chait BT, King RA, Kang JY
Nat Commun (2022) 13 pp. 4668-4668 [ Pubmed: 35970830 DOI: doi:10.1038/s41467-022-32315-y ]
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Rna (nun gene and immunity region) (30 kDa, RNA from Shamshuipovirus HK022)
- Non-template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Hk022 putrna-associated e.coli rna polymerase elongation complex (440 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase (Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
cryo-EM structure of HK022 putRNA-less E.coli RNA polymerase elongation complex
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 7xug
Q-score: 0.446
Hwang S, Olinares PDB, Lee J, Kim J, Chait BT, King RA, Kang JY
Nat Commun (2022) 13 pp. 4668-4668 [ Pubmed: 35970830 DOI: doi:10.1038/s41467-022-32315-y ]
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Rna (nun gene and immunity region) (30 kDa, RNA from Shamshuipovirus HK022)
- Non-template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Magnesium ion (24 Da, Ligand)
- Hk022 putrna-less e.coli rna polymerase elongation complex (440 kDa, Complex)
- Dna/rna (Complex)
- Template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- E.coli rna polymerase (Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of sigma70 bound HK022 putRNA-associated E.coli RNA polymerase elongation complex
Resolution: 3.61 Å
EM Method: Single-particle
Fitted PDBs: 7xui
Q-score: 0.416
Hwang S, Olinares PDB, Lee J, Kim J, Chait BT, King RA, Kang JY
Nat Commun (2022) 13 pp. 4668-4668 [ Pubmed: 35970830 DOI: doi:10.1038/s41467-022-32315-y ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli K-12)
- Template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Sigma70 bound hk022 putrna-associated e.coli rna polymerase elongation complex (510 kDa, Complex)
- Rna (122-mer) (39 kDa, RNA from Shamshuipovirus HK022)
- Rna polymerase (Complex from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)